COX1 antibody (N-Term)

Catalog No. ABIN3042632

Product Details anti-COX1 Antibody

Target: COX1 (Cytochrome C Oxidase Subunit I (COX1))

Binding Specificity: AA 2-14, N-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This COX1 antibody is un-conjugated

Application: Western Blotting (WB)

Purpose: Rabbit IgG polyclonal antibody for Cytochrome c oxidase subunit 1(MT-CO1) detection. Tested with WB in Human.

Sequence: FADRWLFSTN HKD

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Cytochrome c oxidase subunit 1(MT-CO1) detection. Tested with WB in Human.
Gene Name: mitochondrially encoded cytochrome c oxidase I
Protein Name: Cytochrome c oxidase subunit 1

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human MTCO1(2-14aa FADRWLFSTNHKD).

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities.
Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for COX1

Target: COX1 (Cytochrome C Oxidase Subunit I (COX1))

Alternative Name: MT-CO1 (COX1 Products)

Synonyms: COX-1 antibody, COI antibody, MTCO1 antibody, COX1 antibody, CoxI antibody, prostaglandin-endoperoxide synthase 1 antibody, cytochrome c oxidase subunit I antibody, cytochrome c oxidase subunit 1 antibody, cytochrome c oxidase 1 antibody, PTGS1 antibody, COX1 antibody, cox1 antibody

Background: Cytochrome c oxidase subunit I(CO1 or MTCO1) is 1 of 3 mitochondrial DNA(mtDNA) encoded subunits(MTCO1, MTCO2, MTCO3) of respiratory Complex IV. Complex IV is located within the mitochondrial inner membrane and is the third and final enzyme of the electron transport chain of mitochondrial oxidative phosphorylation. It is composed of 13 polypeptides. Subunits I, II, and III(MTCO1, MTCO2, MTCO3) are encoded by mtDNA while subunits IV, Va, Vb, VIa, VIb, VIc, VIIa, VIIb, VIIc, and VIII are nuclear encoded. The cytochrome c oxidase family of enzymes have 4 redox centers, 2 hemes and 2 copper centers. In mitochondrial Complex IV, the 2 hemes are a and a3 and the 2 coppers are CuA and CuB. The 2 hemes and CuB are bound to subunit I. Acin-Perez et al.(2003) identified a cell line containing single and double missense mutations in the cytochrome c oxidase(COX) subunit I gene of mouse mitochondrial DNA. And they hypothesized that deleterious mutations can arise and become predominant, cultured cells can maintain several mtDNA haplotypes at stable frequencies, the respiratory chain has little spare COX capacity, and that the size of a cavity in the vicinity of val421 in MTCO1I of animal COX may affect the function of the enzyme.

Synonyms: COI antibody|COX I antibody|COX1_HUMAN antibody|COXI antibody|Cytochrome c oxidase polypeptide I antibody|Cytochrome c oxidase subunit 1 antibody|Cytochrome C Oxidase subunit I antibody|Mitochondrially encoded cytochrome c oxidase I antibody|MT CO1 antibody|MT-CO1 antibody|MTCO 1 antibody|MTCO1 antibody

UniProt: P00395

Pathways: Proton Transport