GM-CSF antibody (AA 18-141)

Catalog No. ABIN3042527

Product Details anti-GM-CSF Antibody

Target: GM-CSF (CSF2) (Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2))

Binding Specificity: AA 18-141

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GM-CSF antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for Granulocyte-macrophage colony-stimulating factor(CSF2) detection. Tested with WB, IHC-P in Mouse.

Brand: Picoband™

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Granulocyte-macrophage colony-stimulating factor(CSF2) detection. Tested with WB, IHC-P in Mouse.
Gene Name: colony stimulating factor 2 (granulocyte-macrophage)
Protein Name: Granulocyte-macrophage colony-stimulating factor

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived mouse GM-CSF recombinant protein (Position: A18-K141). Mouse GM-CSF shares 54% and 70% amino acid (aa) sequences identity with human and rat GM-CSF, respectively.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Mouse, The detection limit for GM-CSF is approximately 1 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Mouse, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

References for anti-GM-CSF antibody (ABIN3042527)

Fang, Shi, Meng, Li, Peng: "The Balance between Conventional DCs and Plasmacytoid DCs Is Pivotal for Immunological Tolerance during Pregnancy in the Mouse." in: Scientific reports, Vol. 6, pp. 26984, (2018) (PubMed).

Target Details for GM-CSF

Target: GM-CSF (CSF2) (Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2))

Alternative Name: CSF2 (CSF2 Products)

Synonyms: GMCSF antibody, GM-CSF antibody, CSF2 antibody, Csfgm antibody, Gm-CSf antibody, MGI-IGM antibody, Gm-csf antibody, Gmcsf antibody, CSF antibody, colony stimulating factor 2 antibody, colony stimulating factor 2 (granulocyte-macrophage) antibody, CSF2 antibody, Csf2 antibody

Background: GM-CSF, Granulocyte-macrophage colony-stimulating factor, is a protein secreted by macrophages, T cells, mast cells, endothelial cells, and fibroblasts. By fluorescence in situ hybridization, the GM-CSF gene is mapped to 5q31.1. GM-CSF is a cytokine that functions as a white blood cell growth factor. GM-CSF stimulates stem cells to produce granulocytes (neutrophils, eosinophils, and basophils) and monocytes. GM-CSF is an essential regulator of neutrophil function.

Synonyms: Burst Promoting Activity antibody|CMCSF antibody|Colony stimulating factor 2 (granulocyte-macrophage) antibody|Colony Stimulating Factor 2 antibody|Colony stimulating factor antibody|Colony-stimulating factor antibody|CSF 2 antibody|CSF Alpha antibody|CSF antibody|CSF2 antibody|CSF2_HUMAN antibody|Eosinophil Colony Stimulating Factor antibody|GM-CSF antibody|GMCSF antibody|Granulocyte Macrophage Colony Stimulating Factor antibody|Granulocyte-macrophage colony-stimulating factor antibody|MGC131935 antibody|MGC138897 antibody|Molgramostin antibody|Pluripoietin Alpha antibody|Sargramostim antibody

UniProt: P01587

Pathways: JAK-STAT Signaling, Cellular Response to Molecule of Bacterial Origin