S100A9 antibody (AA 2-113)

Catalog No. ABIN3042482

Product Details anti-S100A9 Antibody

Target: S100A9 (S100 Calcium Binding Protein A9 (S100A9))

Binding Specificity: AA 2-113

Reactivity: Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This S100A9 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for Protein S100-A9(S100A9) detection. Tested with WB, IHC-P, ELISA in Mouse,Rat.

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Protein S100-A9(S100A9) detection. Tested with WB, IHC-P, ELISA in Mouse,Rat.
Gene Name: S100 calcium binding protein A9
Protein Name: Protein S100-A9

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived mouse S100A9 recombinant protein (Position: A2-K113). Mouse S100A9 shares 59.8% and 78.6% amino acid (aa) sequence identity with human and rat S100A9, respectively.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Mouse
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
ELISA: Concentration: 0.1-0.5 μg/mL, Tested Species: Mouse

Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

References for anti-S100A9 antibody (ABIN3042482)

Liu, Liu, He, Zhang, Liu, Wang, Qiu, Xu, Xiang, Lv: "Differential gene and lncRNA expression in the lower thoracic spinal cord following ischemia/reperfusion-induced acute kidney injury in rats." in: Oncotarget, Vol. 8, Issue 32, pp. 53465-53481, (2017) (PubMed).

Yan, Tian, Wang, Cheng, Xu, Song, Zhang, Zhang: "Age dependent changes in cartilage matrix, subchondral bone mass, and estradiol levels in blood serum, in naturally occurring osteoarthritis in Guinea pigs." in: International journal of molecular sciences, Vol. 15, Issue 8, pp. 13578-95, (2015) (PubMed).

Target Details for S100A9

Target: S100A9 (S100 Calcium Binding Protein A9 (S100A9))

Alternative Name: S100A9 (S100A9 Products)

Synonyms: 60B8AG antibody, CAGB antibody, CFAG antibody, CGLB antibody, L1AG antibody, LIAG antibody, MAC387 antibody, MIF antibody, MRP14 antibody, NIF antibody, P14 antibody, Mrp14 antibody, S100A9 antibody, MRP-14 antibody, p14 antibody, 60B8Ag antibody, AW546964 antibody, BEE22 antibody, Cagb antibody, GAGB antibody, L1Ag antibody, S100 calcium binding protein A9 antibody, S100 calcium binding protein A9 (calgranulin B) antibody, S100A9 antibody, S100a9 antibody

Background: S100 calcium-binding protein A9 (S100A9), also known as migration inhibitory factor-related protein 14 (MRP14) or calgranulin B, is a protein that in humans is encoded by the S100A9 gene. S100-A9 is a member of the S100 family of proteins containing 2 EF hand calcium-binding motifs. And S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in the inhibition of casein kinase.

Synonyms: Leukocyte L1 complex heavy chain antibody|60B8AG antibody|AW546964 antibody|BEE22 antibody|CAGB antibody|Calgranulin B antibody| Calgranulin-B antibody|Calprotectin L1H subunit antibody|CFAG antibody|CGLB antibody|Cystic fibrosis antigen antibody|Cystic fibrosis antigen B antibody|HGNC:10499 antibody|L1AG antibody|Leukocyte L1 complex heavy chain antibody|LIAG antibody|MAC 387 antibody|MAC387 antibody|MIF antibody|Migration inhibitory factor related protein 14 antibody|Migration inhibitory factor-related protein 14 antibody| MRP 14 antibody|MRP 8 antibody|MRP-14 antibody|MRP14 antibody|Myeloid-related protein 14 antibody|NIF antibody| OTTHUMP00000015331 antibody|P14 antibody|Protein S100-A9 antibody|S100 A9 antibody|S100 calcium binding protein A9 antibody|S100 calcium binding protein A9 calgranulin B antibody|S100 calcium-binding protein A9 antibody|S100A9 antibody| S10A9_HUMAN antibody

Gene ID: 20202

UniProt: P31725

Pathways: Transition Metal Ion Homeostasis, Positive Regulation of Endopeptidase Activity, S100 Proteins