IRF4 antibody (AA 272-451)

Catalog No. ABIN3042469

Product Details anti-IRF4 Antibody

Target: IRF4 (Interferon Regulatory Factor 4 (IRF4))

Binding Specificity: AA 272-451

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IRF4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for Interferon regulatory factor 4(IRF4) detection. Tested with WB, IHC-P in Human.

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Interferon regulatory factor 4(IRF4) detection. Tested with WB, IHC-P in Human.
Gene Name: interferon regulatory factor 4
Protein Name: Interferon regulatory factor 4

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human MUM1 recombinant protein (Position: E272-E451). Human MUM1 shares 92% amino acid (aa) sequence identity with mouse MUM1.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, The detection limit for MUM1 is approximately 0.2 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Target Details for IRF4

Target: IRF4 (Interferon Regulatory Factor 4 (IRF4))

Alternative Name: IRF4 (IRF4 Products)

Synonyms: LSIRF antibody, MUM1 antibody, NF-EM5 antibody, AI385587 antibody, IRF-4 antibody, Spip antibody, IRF4 antibody, si:ch73-170d1.2 antibody, irf4 antibody, interferon regulatory factor 4 antibody, interferon regulatory factor 4a antibody, Interferon regulatory factor 4 antibody, PWWP domain-containing protein MUM1 antibody, IRF4 antibody, Irf4 antibody, irf4a antibody, irf4 antibody, LOC612320 antibody, LOC100068881 antibody

Background: Interferon regulatory factor 4 (IRF4), also known as MUM1, is a protein that in humans is encoded by the IRF4 gene. It is located on 6p25.3. IRF4 is a transcription factor, and it is essential for the development of T helper-2 (Th2) cells, IL17 -producing Th17 cells, and IL9 -producing Th9 cells. In melanocytic cells, the IRF4 gene may be regulated by MITF. IRF4 is a transcription factor that has been implicated in acute leukemia. This gene is strongly associated with pigmentation, sensitivity of skin to sun exposure, freckles, blue eyes, and brown hair color. What's more, IRF4 inhibition is toxic to myeloma cell lines, regardless of transforming oncogenic mechanism.

Synonyms: Interferon regulatory factor 4 antibody|IRF 4 antibody|IRF-4 antibody|Irf4 antibody|IRF4_HUMAN antibody|LSIRF antibody|Lymphocyte specific interferon regulatory factor antibody|Lymphocyte specific IRF antibody|Lymphocyte-specific interferon regulatory factor antibody|Multiple myeloma oncogene 1 antibody|MUM 1 antibody|MUM1 antibody|NF EM5 antibody|NF-EM5 antibody|NFEM5 antibody|PU.1 interaction partner antibody|Sfpi1/PU.1 interaction partner antibody|Transcriptional activator PIP antibody

Gene ID: 3662

UniProt: Q15306

Pathways: Chromatin Binding