HNF1A antibody (AA 431-631)

Catalog No. ABIN3042451

Product Details anti-HNF1A Antibody

Target: HNF1A (HNF1 Homeobox A (HNF1A))

Binding Specificity: AA 431-631

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HNF1A antibody is un-conjugated

Application: Western Blotting (WB)

Purpose: Rabbit IgG polyclonal antibody for Hepatocyte nuclear factor 1-alpha(HNF1A) detection. Tested with WB in Human,Rat.

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Hepatocyte nuclear factor 1-alpha(HNF1A) detection. Tested with WB in Human,Rat.
Gene Name: HNF1 homeobox A
Protein Name: Hepatocyte nuclear factor 1-alpha

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human HNF1 recombinant protein (Position: A431-Q631). Human HNF1 shares 93% and 92% amino acid (aa) sequences identity with mouse and rat HNF1, respectively.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat, The detection limit for HNF1 is approximately 0.25 ng/lane under reducing conditions.
Notes: Tested Species: Species with positive results.
Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

References for anti-HNF1A antibody (ABIN3042451)

Dai, Cai, Wu, Chen, Han: "Protocatechuic acid inhibits hepatitis B virus replication by activating ERK1/2 pathway and down-regulating HNF4α and HNF1α in vitro." in: Life sciences, Vol. 180, pp. 68-74, (2017) (PubMed).

Target Details for HNF1A

Target: HNF1A (HNF1 Homeobox A (HNF1A))

Alternative Name: HNF1A (HNF1A Products)

Synonyms: HNF-1A antibody, HNF1 antibody, IDDM20 antibody, LFB1 antibody, MODY3 antibody, TCF-1 antibody, TCF1 antibody, HNF-1a antibody, AI323641 antibody, HNF1-alpha antibody, HNF1[a] antibody, Hnf-1 antibody, Hnf1alpha antibody, Tcf-1 antibody, Tcf1 antibody, HNF-1-alpha antibody, HNF-1alpha antibody, LF-B1 antibody, Lfb1 antibody, HNF-1 antibody, HNF1A antibody, tcf1 antibody, FB1b antibody, HNF1b antibody, XLFB1b1 antibody, hnf1-B antibody, lfb1b antibody, mody3 antibody, tcf1-B antibody, tcfb-A antibody, HNF-1-alpha-A antibody, HNF-1A-A antibody, LFB1a1 antibody, TCF-1-A antibody, XLFB1 antibody, XLFB1b antibody, hnf1-a antibody, hnf1a antibody, hnf1a-a antibody, hnf1a-b antibody, hnf1a.L antibody, tcf1-A antibody, xx:af244934-22s antibody, HNF1 homeobox A antibody, HNF1 homeobox A L homeolog antibody, HNF1 homeobox A S homeolog antibody, HNF1 homeobox a antibody, HNF1A antibody, Hnf1a antibody, hnf1a antibody, hnf1a.L antibody, hnf1a.S antibody

Background: HNF1 homeobox A (hepatocyte nuclear factor 1 homeobox A), also known as HNF1A or TCF1, is a human gene. It is mapped to chromosome 12q24.3. The protein encoded by this gene is a transcription factor that is highly expressed in the liver and is involved in the regulation of the expression of several liver-specific genes. HNF1A can function as a dimer. What's more, deletion of the HNF1A activation domains or interruption of the HNF1-binding sites in the LCT promoter resulted in complete loss of transcriptional activity.

Synonyms: Albumin proximal factor antibody|Hepatic nuclear factor 1 alpha antibody|Hepatic nuclear factor 1 antibody|Hepatic transcription factor 1 alpha antibody|Hepatic transcription factor 1 antibody|Hepatocyte nuclear factor 1-alpha antibody|HNF 1 antibody|HNF 1A antibody|HNF-1-alpha antibody|HNF-1A antibody|HNF1A antibody|HNF1A_HUMAN antibody|Interferon production regulator factor antibody|LF B1 antibody|LF B1 hepatic nuclear factor antibody|LFB 1 antibody|LFB1 antibody|LFB1 hepatic nuclear factor antibody|Liver specific transcription factor LF B1 antibody|Liver specific transcription factor LFB1 antibody|Liver-specific transcription factor LF-B1 antibody|Maturity onset diabetes of the young 3 antibody|MODY 3 antibody|MODY3 antibody|TCF 1 antibody|TCF-1 antibody|TCF1 antibody|Transcription factor 1 antibody|Transcription factor 1 hepatic antibody

Gene ID: 6927

UniProt: P20823

Pathways: Hormone Transport, Carbohydrate Homeostasis