HEXB antibody (AA 381-556)

Catalog No. ABIN3042442

Product Details anti-HEXB Antibody

Target: HEXB (Hexosaminidase B (Beta Polypeptide) (HEXB))

Binding Specificity: AA 381-556

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HEXB antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for Beta-hexosaminidase subunit beta(HEXB) detection. Tested with WB, IHC-P in Human.

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Beta-hexosaminidase subunit beta(HEXB) detection. Tested with WB, IHC-P in Human.
Gene Name: hexosaminidase B (beta polypeptide)
Protein Name: Beta-hexosaminidase subunit beta

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human HEXB recombinant protein (Position: K381-M556). Human HEXB shares 75% and 73% amino acid (aa) sequences identity with mouse and rat HEXB, respectively.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, The detection limit for HEXB is approximately 0.2 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Target Details for HEXB

Target: HEXB (Hexosaminidase B (Beta Polypeptide) (HEXB))

Alternative Name: HEXB (HEXB Products)

Synonyms: ENC-1AS antibody, hexosaminidase B antibody, hexosaminidase subunit beta antibody, Hexb antibody, HEXB antibody

Background: Beta-hexosaminidase subunit beta (HEXB) is an enzyme that in humans is encoded by the HEXB gene. It is mapped to 5q13.3. HEXB is the beta subunit of the lysosomal enzyme beta-hexosaminidase that, together with the cofactor GM2 activator protein, catalyzes the degradation of the ganglioside GM2, and other molecules containing terminal N-acetyl hexosamines. Beta subunit gene mutations lead to Sandhoff disease (GM2-gangliosidosis type II). It has been found that HEXB is a peptidoglycan hydrolase and it is involved in restricting mycobacteria growth even before the onset of adaptive immunity.

Synonyms: Beta hexosaminidase beta chain antibody|Beta hexosaminidase subunit beta antibody|Beta N acetylhexosaminidase antibody|Beta-hexosaminidase subunit beta chain A antibody|Beta-N-acetylhexosaminidase subunit beta antibody|Cervical cancer proto oncogene 7 protein antibody|Cervical cancer proto-oncogene 7 protein antibody|ENC 1AS antibody|HCC 7 antibody|HCC-7 antibody|HCC7 antibody|HEX B antibody|Hexb antibody|HEXB_HUMAN antibody|Hexosaminidase B (beta polypeptide) antibody|Hexosaminidase B antibody|Hexosaminidase subunit B antibody|HexosaminidaseB antibody|N acetyl beta glucosaminidase antibody|N-acetyl-beta-glucosaminidase subunit beta antibody

Gene ID: 3074

UniProt: P07686

Pathways: Sensory Perception of Sound, Glycosaminoglycan Metabolic Process