Gelsolin antibody (AA 580-782)

Catalog No. ABIN3042436

Product Details anti-Gelsolin Antibody

Target: Gelsolin (GSN)

Binding Specificity: AA 580-782

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Gelsolin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for Gelsolin(GSN) detection. Tested with WB, IHC-P in Human,Mouse,Rat.

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Gelsolin(GSN) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: gelsolin
Protein Name: Gelsolin

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human Gelsolin recombinant protein (Position: E580-A782). Human Gelsolin shares 94% and 95% amino acid (aa) sequences identity with mouse and rat Gelsolin, respectively.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, The detection limit for Gelsolin is approximately 0.25 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

References for anti-Gelsolin antibody (ABIN3042436)

Guo, Cui, Jiang, Zhang, Jiang: "Proteomics of acute heart failure in a rat post-myocardial infarction model." in: Molecular medicine reports, Vol. 16, Issue 2, pp. 1946-1956, (2018) (PubMed).

Target Details for Gelsolin

Target: Gelsolin (GSN)

Alternative Name: GSN (GSN Products)

Synonyms: ADF antibody, AGEL antibody, CG1106 antibody, DGS antibody, Dmel\\CG1106 antibody, gel antibody, scin antibody, cb107 antibody, gsn antibody, sb:cb107 antibody, u-gelsolin antibody, wu:fi16f06 antibody, gelsolin antibody, Gelsolin antibody, gelsolin S homeolog antibody, gelsolin a antibody, GSN antibody, Gel antibody, Gsn antibody, gsn.S antibody, gsn antibody, gsna antibody

Background: Gelsolin, also known as GNS or brevin, is an actin-binding protein that is a key regulator of actin filament assembly and disassembly. Gelsolin is one of the most potent members of the actin-severing gelsolin/villin superfamily. The gene was assigned to human chromosome 9q33.2. It is the principal intracellular and extracellular actin-severing protein. Gelsolin and Gc protein together constitute the extracellular actin-scavenger system which prevents the toxic effects of actin release into the extracellular space under circumstances of cell necrosis. Gelsolin may have therapeutic potential as a mucolytic agent in CF patients. The antiapoptotic activity of gelsolin seems to prevent a step leading to cytochrome c release from the mitochondria into the cytosol.

Synonyms: Actin depolymerizing factor antibody|Actin-depolymerizing factor antibody|ADF antibody|AGEL antibody|Brevin antibody|DKFZp313L0718 antibody|GELS_HUMAN antibody|Gelsolin antibody|GSN antibody

Gene ID: 2934

UniProt: P06396

Pathways: Caspase Cascade in Apoptosis, Regulation of Actin Filament Polymerization, Autophagy