GFRA1 antibody (AA 25-227)

Catalog No. ABIN3042422

Product Details anti-GFRA1 Antibody

Target: GFRA1 (GDNF Family Receptor alpha 1 (GFRA1))

Binding Specificity: AA 25-227

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GFRA1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for GDNF family receptor alpha-1(GFRA1) detection. Tested with WB, IHC-P in Human.

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for GDNF family receptor alpha-1(GFRA1) detection. Tested with WB, IHC-P in Human.
Gene Name: GDNF family receptor alpha 1
Protein Name: GDNF family receptor alpha-1

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human GFRA1 recombinant protein (Position: D25-Q227). Human GFRA1 shares 97% amino acid (aa) sequence identity with both mouse and rat GFRA1.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, The detection limit for GFRA1 is approximately 0.2 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Target Details for GFRA1

Target: GFRA1 (GDNF Family Receptor alpha 1 (GFRA1))

Alternative Name: GFRA1 (GFRA1 Products)

Synonyms: gdnfr antibody, ret1l antibody, retl1 antibody, trnr1 antibody, gdnfra antibody, MGC122616 antibody, gfr-alpha-1 antibody, GDNFR antibody, GDNFRA antibody, GFR-ALPHA-1 antibody, RET1L antibody, RETL1 antibody, TRNR1 antibody, GDNFRALPHA antibody, GFRA1 antibody, AU042498 antibody, gfralpha1a antibody, zgc:110129 antibody, GDNF family receptor alpha 1 antibody, glial cell line derived neurotrophic factor family receptor alpha 1 antibody, gdnf family receptor alpha 1a antibody, gfra1 antibody, GFRA1 antibody, Gfra1 antibody, gfra1a antibody

Background: GDNF family receptor alpha-1 (GFRα1), also known as the GDNF receptor or GFRA1, is a protein that in humans is encoded by the GFRA1 gene. It is mapped to chromosome 10q25.3. The protein encoded by this gene is a member of the GDNF receptor family. GFRA1 is released by neuronal cells, Schwann cells, and injured sciatic nerve. It is a glycosylphosphatidylinositol(GPI)-linked cell surface receptor for both GDNF and NTN, and mediates activation of the RET tyrosine kinase receptor. This gene is also a candidate gene for Hirschsprung disease. Soluble GFRA1 mediates robust recruitment of RET to lipid rafts via a mechanism requiring the RET tyrosine kinase.

Synonyms: GDNF Family Receptor Alpha 1 antibody|GDNF R antibody|GDNF RA antibody|GDNF receptor alpha antibody|GDNFR alpha 1 antibody|GDNFR alpha antibody|GDNFR antibody|GDNFRA antibody|GDNFRalpha antibody|GFR alpha 1 antibody|GFR alpha1 antibody|GFR-ALPHA-1 antibody|GFRA 1 antibody|GFRA1 antibody|GFRalpha1 antibody|Glial Cell Line DErived Neurotrophic Factor Receptor Alpha antibody|Glial cell line-derived neurotrophic factor receptor alpha antibody|GPI linked anchor protein antibody|GPI-linked anchor protein antibody|MGC23045 antibody|PI linked cell surface accessory protein antibody|RET 1L antibody|RET ligand 1 antibody|RET1L antibody|RETL 1 antibody|RETL1 antibody|TGF beta related neurotrophic factor receptor 1 antibody|TGF-beta related neurotrophic factor receptor 1 antibody|TRNR 1 antibody|TRNR1 antibody

Gene ID: 14585

UniProt: P56159