Glucagon antibody (N-Term)

Catalog No. ABIN3042419

Product Details anti-Glucagon Antibody

Target: Glucagon (GCG)

Binding Specificity: AA 53-81, N-Term

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Glucagon antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for Glucagon(GCG) detection. Tested with IHC-P in Human,Mouse,Rat.

Sequence: HSQGTFTSDY SKYLDSRRAQ DFVQWLMNT

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Glucagon(GCG) detection. Tested with IHC-P in Human,Mouse,Rat.
Gene Name: glucagon
Protein Name: Glucagon

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human GLP1 (53-81aa HSQGTFTSDYSKYLDSRRAQDFVQWLMNT), identical to the related mouse and rat sequences.

Isotype: IgG

Application Details

Application Notes: IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

References for anti-Glucagon antibody (ABIN3042419)

Yang, Yan, Tong, Zhang, Chen, Xue, Wang, Chu, Jin, Li: "Chronic oscillating glucose challenges disarrange innate immune homeostasis to potentiate the variation of neutrophil-lymphocyte ratio in rats with or without hidden diabetes mellitus." in: Diabetes, metabolic syndrome and obesity : targets and therapy, Vol. 11, pp. 277-288, (2018) (PubMed).

Wang, Tian, Chen, Tan, Xie, Chen, Fu, Chen, Xu, Wei: "Low dose doxycycline decreases systemic inflammation and improves glycemic control, lipid profiles, and islet morphology and function in db/db mice." in: Scientific reports, Vol. 7, Issue 1, pp. 14707, (2017) (PubMed).

Zhang, Ye, Wang, Gao, Tan, Zhuo, Liu, Xia, Yang, Li: "Kidney-targeted transplantation of mesenchymal stem cells by ultrasound-targeted microbubble destruction promotes kidney repair in diabetic nephropathy rats." in: BioMed research international, Vol. 2013, pp. 526367, (2013) (PubMed).

Tang, Zhu, Fan, Zhang, Li, Liu, Chen, Zhang, Zhou, Wei: "Intra-arterial targeted islet-specific expression of Sirt1 protects ? cells from streptozotocin-induced apoptosis in mice." in: Molecular therapy : the journal of the American Society of Gene Therapy, Vol. 19, Issue 1, pp. 60-6, (2011) (PubMed).

Lan, Yue, Han, Shi, Yang, Pu, Yao, Kang: "Genome-wide identification of TCF7L2/TCF4 target miRNAs reveals a role for miR-21 in Wnt-driven epithelial cancer." in: International journal of oncology, Vol. 40, Issue 2, pp. 519-26, (2011) (PubMed).

Chen, Yang, Zheng, Hu, Kong, Zhang: "Effect of catch-up growth after food restriction on the entero-insular axis in rats." in: Nutrition & metabolism, Vol. 7, pp. 45, (2010) (PubMed).

Target Details for Glucagon

Target: Glucagon (GCG)

Alternative Name: GCG (GCG Products)

Synonyms: GLP1 antibody, GLP2 antibody, GRPP antibody, GLP-1 antibody, Glu antibody, PPG antibody, GCG antibody, gcg-A antibody, gcg1 antibody, glucagon antibody, glucagon L homeolog antibody, GCG antibody, Gcg antibody, gcg.L antibody

Background: GCG is also known as GLP1, or Glucagon. Glucagon is a 29-amino acid pancreatic hormone that counteracts the glucose-lowering action of insulin by stimulating glycogenolysis and gluconeogenesis. It is mapped to 2q36-2q37. GLP1, also known as 7-37 for the codons of the preproglucagon molecule which encode it, renders pancreatic beta-cells 'glucose-competent' and may be useful in the treatment of noninsulin-dependent diabetes mellitus. Also, GLP1 is a potent insulin secretagogue. It plays a major role in the enteroinsular axis, accounting, for example, for the finding that plasma insulin levels accompanying oral intake of glucose are greater than those observed when glucose is given intravenously.

Synonyms: GCG antibody|glicentin-related polypeptide antibody|GLP-1 antibody|GLP-1(7-36) antibody|GLP-1(7-37) antibody|GLP-2 antibody|GLP1 antibody|GLP1, included antibody|GLP2 antibody|GLP2, included antibody|GLUC_HUMAN antibody|Glucagon antibody|Glucagon like peptide 1 antibody|glucagon-like peptide 1 antibody|Glucagon-like peptide 1, included antibody|Glucagon-like peptide 2 antibody|Glucagon-like peptide 2, included antibody|GRPP antibody|OXM antibody|OXY antibody|preproglucagon antibody

Gene ID: 2641

UniProt: P01275

Pathways: Positive Regulation of Peptide Hormone Secretion, Peptide Hormone Metabolism, cAMP Metabolic Process, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Negative Regulation of intrinsic apoptotic Signaling