FOXP3 antibody (AA 101-431)

Catalog No. ABIN3042405

Product Details anti-FOXP3 Antibody

Target: FOXP3 (Forkhead Box P3 (FOXP3))

Binding Specificity: AA 101-431

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FOXP3 antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for Forkhead box protein P3(FOXP3) detection. Tested with IHC-P in Human,Mouse,Rat.

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Forkhead box protein P3(FOXP3) detection. Tested with IHC-P in Human,Mouse,Rat.
Gene Name: forkhead box P3
Protein Name: Forkhead box protein P3

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human FOXP3 recombinant protein (Position: H101-P431). Human FOXP3 shares 88% amino acid (aa) sequence identity with mouse FOXP3.

Isotype: IgG

Application Details

Application Notes: IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

References for anti-FOXP3 antibody (ABIN3042405)

Li, Chen, Xie: "Decreased FOXP3+ and GARP+ Tregs to neoadjuvant chemotherapy associated with favorable prognosis in advanced gastric cancer." in: OncoTargets and therapy, Vol. 9, pp. 3525-33, (2016) (PubMed).

Tao, Zhu, Chen, Wang, Pan, Yu, Zhou, Zhu: "IL-35 improves Treg-mediated immune suppression in atherosclerotic mice." in: Experimental and therapeutic medicine, Vol. 12, Issue 4, pp. 2469-2476, (2016) (PubMed).

Gong, Wang, Yuan, Li, Gu, Zhao, Zhang, Jia, Feng, Liu: "Inhibition of Tumor Growth and Immunomodulatory Effects of Flavonoids and Scutebarbatines of Scutellaria barbata D. Don in Lewis-Bearing C57BL/6 Mice." in: Evidence-based complementary and alternative medicine : eCAM, Vol. 2015, pp. 630760, (2015) (PubMed).

Li, Ren, Wang, Gu, Hu, Ren, Hong, Wu, Liu, Li: "T2 enhances in situ level of Foxp3+ regulatory cells and modulates inflammatory cytokines in Crohn's disease." in: International immunopharmacology, Vol. 18, Issue 2, pp. 244-8, (2014) (PubMed).

Wang, Dai, Dong, Sun, Song, Guo, Zhu, Wang, Zhang: "The modulation of endoplasmic reticulum stress by chemical chaperone upregulates immune negative cytokine IL-35 in apolipoprotein E-deficient mice." in: PLoS ONE, Vol. 9, Issue 1, pp. e87787, (2014) (PubMed).

Gai, Li, Song, Lei, Yang: "In situ analysis of FOXP3(+) regulatory T cells and myeloid dendritic cells in human colorectal cancer tissue and tumor-draining lymph node." in: Biomedical reports, Vol. 1, Issue 2, pp. 207-212, (2014) (PubMed).

Hu, Li, Maskey, Xu, Peng, Wang, Li, Yang: "Decreased intratumoral Foxp3 Tregs and increased dendritic cell density by neoadjuvant chemotherapy associated with favorable prognosis in advanced gastric cancer." in: International journal of clinical and experimental pathology, Vol. 7, Issue 8, pp. 4685-94, (2014) (PubMed).

Qiao, Huang, Xu, Zhao, Ran, Shen: "LncRNA DQ786243 affects Treg related CREB and Foxp3 expression in Crohn's disease." in: Journal of biomedical science, Vol. 20, pp. 87, (2014) (PubMed).

Fu, Li, Yang, Gai, Jia, Lei, Li: "FOXP3 and TLR4 protein expression are correlated in non-small cell lung cancer: implications for tumor progression and escape." in: Acta histochemica, Vol. 115, Issue 2, pp. 151-7, (2013) (PubMed).

Gai, Song, Li, Lei, Yang: "Potential role of plasmacytoid dendritic cells for FOXP3+ regulatory T cell development in human colorectal cancer and tumor draining lymph node." in: Pathology, research and practice, Vol. 209, Issue 12, pp. 774-8, (2013) (PubMed).

Target Details for FOXP3

Target: FOXP3 (Forkhead Box P3 (FOXP3))

Alternative Name: FOXP3 (FOXP3 Products)

Synonyms: foxp3 antibody, FOXP3 antibody, AIID antibody, DIETER antibody, IPEX antibody, PIDX antibody, XPID antibody, JM2 antibody, scurfin antibody, sf antibody, RGD1562112 antibody, forkhead box P3 antibody, forkhead box P3a antibody, forkhead box P3 L homeolog antibody, FOXP3 antibody, foxp3a antibody, foxp3 antibody, foxp3.L antibody, Foxp3 antibody

Background: FOXP3(forkhead box P3) is a protein involved in immune system responses. The human FOXP3 genes contain 11 coding exons. Exon-intron boundaries are identical across the coding regions of the mouse and human genes. By genomic sequence analysis, the FOXP3 gene maps to the p arm of the X chromosome(specifically, Xp11.23). A member of the FOX protein family, FOXP3 appears to function as a master regulator in the development and function of regulatory T cells. While the precise control mechanism has not yet been established, FOX proteins belong to the forkhead/winged-helix family of transcriptional regulators and are presumed to exert control via similar DNA binding interactions during transcription.

Synonyms: AIID antibody|DIETER antibody|Forkhead box P3 antibody|Forkhead box protein P3 antibody|foxp3 antibody|FOXP3_HUMAN antibody|FOXP3delta7 antibody|Immune dysregulation polyendocrinopathy enteropathy X linked antibody|Immunodeficiency polyendocrinopathy enteropathy X linked antibody|IPEX antibody|JM2 antibody|MGC141961 antibody|MGC141963 antibody|OTTHUMP00000025832 antibody|OTTHUMP00000025833 antibody|OTTHUMP00000226737 antibody|PIDX antibody|SCURFIN antibody|XPID antibody

Gene ID: 50943

Pathways: Chromatin Binding, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation