Flotillin 2 antibody (AA 169-344)

Catalog No. ABIN3042393

Product Details anti-Flotillin 2 Antibody

Target: Flotillin 2 (FLOT2)

Binding Specificity: AA 169-344

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Flotillin 2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for Flotillin-2(FLOT2) detection. Tested with WB, IHC-P in Human,Mouse,Rat.

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Flotillin-2(FLOT2) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: flotillin 2
Protein Name: Flotillin-2

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human Flotillin 2 recombinant protein (Position: K169-K344). Human Flotillin 2 shares 100% amino acid (aa) sequence identity with both mouse and rat Flotillin 2.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, Rat
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Target Details for Flotillin 2

Target: Flotillin 2 (FLOT2)

Alternative Name: FLOT2 (FLOT2 Products)

Synonyms: ECS-1 antibody, ECS1 antibody, ESA antibody, ESA1 antibody, M17S1 antibody, AI573412 antibody, Esa antibody, reggie-2 antibody, CG11547 antibody, CG14409 antibody, CG32593 antibody, DmFlo2 antibody, Dmel\\CG32593 antibody, FLO2 antibody, FLO[Dm]-2 antibody, Flo2 antibody, flo2 antibody, reggie-1 antibody, ecs-1 antibody, ecs1 antibody, esa antibody, esa1 antibody, flotillin-2 antibody, FLOT2 antibody, fb48a04 antibody, im:7143869 antibody, wu:fb48a04 antibody, zgc:55821 antibody, flotillin 2 antibody, Flotillin 2 antibody, flotillin 2 L homeolog antibody, flotillin 2a antibody, FLOT2 antibody, Flot2 antibody, Flo2 antibody, flot2.L antibody, flot2 antibody, flot2a antibody

Background: FLOT2 (Flotillin 2), also known as ESA1 or M17S1, is a protein that in humans is encoded by the FLOT2 gene. Schroeder et al. (1991) isolated a cDNA for an epidermal surface antigen believed to be involved in epidermal cell adhesion. By analysis of a somatic cell hybrid panel and in situ hybridization using the ESA cDNA, the gene was mapped to 17q11-q12 in the region containing the NF1 gene. Bickel et al. (1997) found that mouse Flot2 consistently copurifies with Flot1 and with caveolin-1 in the purification of caveolin-rich membranes. Using a quantitative proteomic analysis of cultured mouse neuronal stem cells, Li et al. (2012) found that palmitoylation and oligomerization of flotillin-2 was abolished in homozygous Dhhc5 mutant neuronal stem cells. The absolute amount of flotillin-2 was not changed in Dhhc5 mutant neurons.

Synonyms: ECS-1 antibody|ECS1 antibody|Epidermal surface antigen 1 antibody|Epidermal surface antigen antibody|ESA antibody|ESA1 antibody|Flot2 antibody|FLOT2_HUMAN antibody|Flotillin-2 antibody|M17S1 antibody|Membrane component chromosome 17 surface marker 1 antibody|Membrane component chromosome 17 surface marker 1 homolog antibody|Membrane component, chromosome 17, surface marker 1 antibody|REG-1 antibody| Reggie-1 antibody|Reggie-2 antibody

Gene ID: 2319

UniProt: Q14254