Myeloperoxidase antibody (AA 406-745)

Catalog No. ABIN3042368

Product Details anti-Myeloperoxidase Antibody

Target: Myeloperoxidase (MPO)

Binding Specificity: AA 406-745

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Myeloperoxidase antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for Myeloperoxidase(MPO) detection. Tested with WB, IHC-P in Human,Mouse,Rat.

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Myeloperoxidase(MPO) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: myeloperoxidase
Protein Name: Myeloperoxidase

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human Myeloperoxidase recombinant protein (Position:S406-S745). Human Myeloperoxidase shares 90% amino acid (aa) sequence identity with mouse Myeloperoxidase.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, The detection limit for Myeloperoxidase is approximately 0.25 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

References for anti-Myeloperoxidase antibody (ABIN3042368)

Wei, Kong, Li, Guan, Wang, Miao: "Nicotinamide mononucleotide attenuates brain injury after intracerebral hemorrhage by activating Nrf2/HO-1 signaling pathway." in: Scientific reports, Vol. 7, Issue 1, pp. 717, (2018) (PubMed).

Xu, Zhang, He, Li, Gao, Liu: "Lactobacillus acidophilus alleviates pouchitis after ileal pouch-anal anastomosis in rats." in: World journal of gastroenterology, Vol. 23, Issue 26, pp. 4735-4743, (2018) (PubMed).

Wang, Liu, Liu, Wang, Fan, Ma, Jin, Xiang, Huang, Zhang, Xu, Yin, He: "Interleukin-17A Aggravates Middle Ear Injury Induced by Streptococcus pneumoniae through the p38 Mitogen-Activated Protein Kinase Signaling Pathway." in: Infection and immunity, Vol. 85, Issue 10, (2017) (PubMed).

Yu, Chen, Wang, Kuang, Liu, Zhang, Du: "Neuroprotective effect of kaempferol glycosides against brain injury and neuroinflammation by inhibiting the activation of NF-?B and STAT3 in transient focal stroke." in: PLoS ONE, Vol. 8, Issue 2, pp. e55839, (2013) (PubMed).

Target Details for Myeloperoxidase

Target: Myeloperoxidase (MPO)

Alternative Name: MPO (MPO Products)

Synonyms: mKIAA4033 antibody, MPO antibody, LOC100335032 antibody, POX2' antibody, XPOX2' antibody, mpo antibody, mpo-A antibody, pmr-1 antibody, pmr1 antibody, pox2 antibody, xpox2 antibody, myeloperoxidase antibody, myeloperoxidase L homeolog antibody, MPO antibody, Mpo antibody, LOC100335032 antibody, mpo.L antibody

Background: Myeloperoxidase (MPO) is a mammalian phagocyte hemoprotein thought to primarily mediate host defense reactions. It is abundantly expressed in neutrophils and secreted during their activation. Myeloperoxidase is part of the host defense system of human polymorphonuclear leukocytes, responsible for microbicidal activity against a wide range of organisms. It is located in the nucleus as well as in the cytoplasm. Intranuclear MPO may help to protect DNA against damage resulting from oxygen radicals produced during myeloid cell maturation and function. The standard product used in this kit is the product of gene recombination, consisting of 697(A49-S745) amino acids with the molecular mass of 80KDa.

Synonyms: 84 kDa myeloperoxidase antibody|89 kDa myeloperoxidase antibody|EC 1.11.1.7 antibody|EC1.11.2.2 antibody|MPO antibody|Myeloperoxidase heavy chain antibody|Myeloperoxidase light chain antibody|PERM_HUMAN antibody

Gene ID: 4353

UniProt: P05164

Pathways: Chromatin Binding