Ubiquitin B antibody (AA 77-152)

Catalog No. ABIN3042338

Product Details anti-Ubiquitin B Antibody

Target: Ubiquitin B (UBB)

Binding Specificity: AA 77-152

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Ubiquitin B antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for Ubiquitin-60S ribosomal protein L40(UBA52) detection. Tested with WB, IHC-P in Human,Mouse,Rat.

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Ubiquitin-60S ribosomal protein L40(UBA52) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: ubiquitin B
Protein Name: Polyubiquitin-B

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human Ubiquitin recombinant protein (Position: M77-G152).

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, The detection limit for Ubiquitin is approximately 0.25 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Target Details for Ubiquitin B

Target: Ubiquitin B (UBB)

Alternative Name: UBB (UBB Products)

Synonyms: AL033289 antibody, Rps27a antibody, Uba52 antibody, Ubb2 antibody, Ubc antibody, UBC antibody, Ubiquitin antibody, UBB antibody, Ubi-p63E antibody, im:6892314 antibody, si:ch211-202a12.3 antibody, ubc antibody, zUBC antibody, zgc:172187 antibody, ubiquitin B antibody, ubiquitin C antibody, UBB antibody, Ubb antibody, UBC antibody, ubb antibody

Background: Ubiquitin (originally, ubiquitous immunopoietic polypeptide) is a small regulatory protein that has been found in almost all tissues (ubiquitously) of eukaryotic organisms. Its gene is mapped to 16p 13.3. The ubiquitin protein itself consists of 76 amino acids and has a molecular mass of about 8.5 kDa. Ubiquitin binds to proteins and labels them for destruction. It directs protein recycling and also direct proteins to other locations in the cell, where they control other protein and cell mechanisms.

Synonyms: FLJ25987 antibody|MGC8385 antibody|Polyubiquitin B antibody|RPS 27A antibody|RPS27A antibody|UBA 52 antibody|UBA 80 antibody|UBA52 antibody|UBA80 antibody|UBB antibody|UBB_HUMAN antibody|UBC antibody|UBCEP 1 antibody|UBCEP 2 antibody|UBCEP1 antibody|UBCEP2 antibody|Ubiquitin antibody|ubiquitin B antibody

Gene ID: 7314

Pathways: Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Mitotic G1-G1/S Phases, DNA Replication, Toll-Like Receptors Cascades, Synthesis of DNA, Autophagy, EGFR Downregulation, Ubiquitin Proteasome Pathway