VWF antibody (AA 2535-2813)

Catalog No. ABIN3042328

Product Details anti-VWF Antibody

Target: VWF (Von Willebrand Factor (VWF))

Binding Specificity: AA 2535-2813

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This VWF antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for von Willebrand factor(VWF) detection. Tested with WB, IHC-P in Human.

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for von Willebrand factor(VWF) detection. Tested with WB, IHC-P in Human.
Gene Name: von Willebrand factor
Protein Name: von Willebrand factor

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human VWF recombinant protein (Position: R2535-K2813). Human VWF shares 79% amino acid (aa) sequence identity with mouse VWF.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, The detection limit for VWF is approximately 0.25 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

References for anti-VWF antibody (ABIN3042328)

Zhang, Zhao, Zhao, Gu, Peng, Pu, Wu: "Expression and correlation analysis of RegIV and vascular endothelial growth factors (VEGF-A and VEGF-C) in metastatic spinal tumors." in: Oncology letters, Vol. 13, Issue 5, pp. 3517-3521, (2017) (PubMed).

Zhang, Li, Zheng, Lin, Zhang, Zhang: "Inhibition of angiogenesis by arsenic trioxide via TSP-1-TGF-β1-CTGF-VEGF functional module in rheumatoid arthritis." in: Oncotarget, Vol. 8, Issue 43, pp. 73529-73546, (2017) (PubMed).

Mu, Song, Zhang, Lin, Dong: "Calcium signaling is implicated in the diffuse axonal injury of brain stem." in: International journal of clinical and experimental pathology, Vol. 8, Issue 5, pp. 4388-97, (2016) (PubMed).

Ding, Chen, Xu, Qin: "Effect of Hyperbaric Oxygen on the Growth of Intracranial Glioma in Rats." in: Chinese medical journal, Vol. 128, Issue 23, pp. 3197-203, (2016) (PubMed).

Pan, Yu, Huang, Zhu: "Resveratrol Protects against TNF-α-Induced Injury in Human Umbilical Endothelial Cells through Promoting Sirtuin-1-Induced Repression of NF-KB and p38 MAPK." in: PLoS ONE, Vol. 11, Issue 1, pp. e0147034, (2016) (PubMed).

Yang, Yang, Cao, Man, Li, Xing: "Paxillin regulates vascular endothelial growth factor A-induced in vitro angiogenesis of human umbilical vein endothelial cells." in: Molecular medicine reports, Vol. 11, Issue 3, pp. 1784-92, (2015) (PubMed).

Qi, Li, Zhang, Zhang, Wang, Zhang, Gui, Huang: "Correction: IFI6 Inhibits Apoptosis via Mitochondrial-Dependent Pathway in Dengue Virus 2 Infected Vascular Endothelial Cells." in: PLoS ONE, Vol. 10, Issue 9, pp. e0138896, (2015) (PubMed).

Wang, Jiang, Wang, Li, Zhang, Zhao, Peng, Cao, Sun: "Construction of tissue-engineered bone using a bioreactor and platelet-rich plasma." in: Experimental and therapeutic medicine, Vol. 8, Issue 2, pp. 413-418, (2014) (PubMed).

Zhu, Tuerxun, Hui, Abliz: "Effects of propranolol and isoproterenol on infantile hemangioma endothelial cells in vitro." in: Experimental and therapeutic medicine, Vol. 8, Issue 2, pp. 647-651, (2014) (PubMed).

Wu, Chen, Guo, He, Hu: "Effects of transforming growth factor-?2 on myocilin expression and secretion in human primary cultured trabecular meshwork cells." in: International journal of clinical and experimental pathology, Vol. 7, Issue 8, pp. 4827-36, (2014) (PubMed).

Yang, Qin, Wang, Chen, Yuan, Fang, Yao, Jiao, Yu, Zhang, Wang: "Pinocembrin, a major flavonoid in propolis, improves the biological functions of EPCs derived from rat bone marrow through the PI3K-eNOS-NO signaling pathway." in: Cytotechnology, Vol. 65, Issue 4, pp. 541-51, (2013) (PubMed).

Han, Jia, Dai, Yang, Zhao, Zhao, Fan, Yuan: "Performance of a multilayered small-diameter vascular scaffold dual-loaded with VEGF and PDGF." in: Biomaterials, Vol. 34, Issue 30, pp. 7302-13, (2013) (PubMed).

Liu, Zhang, Hou, Wang, Yang, Guan, Dong, Gao, Feng: "Vascularized bone tissue formation induced by fiber-reinforced scaffolds cultured with osteoblasts and endothelial cells." in: BioMed research international, Vol. 2013, pp. 854917, (2013) (PubMed).

Xu, Fu, Chen, Xiao, Lan, Le, Cheng, He, Shen, Huang, Liu: "Development of an optimized protocol for primary culture of smooth muscle cells from rat thoracic aortas." in: Cytotechnology, Vol. 61, Issue 1-2, pp. 65-72, (2010) (PubMed).

Zhao, Chen, Li, Xu: "PlexinA1 expression in gastric carcinoma and its relationship with tumor angiogenesis and proliferation." in: World journal of gastroenterology, Vol. 13, Issue 48, pp. 6558-61, (2007) (PubMed).

Jiang, Xu, Zhu, Chen, Qian, Qiao, Yang, Wang, Chen: "Histological type of oncogenity and expression of cell cycle genes in tumor cells from human mesenchymal stem cells." in: Oncology reports, Vol. 16, Issue 5, pp. 1021-8, (2006) (PubMed).

Target Details for VWF

Target: VWF (Von Willebrand Factor (VWF))

Alternative Name: VWF (VWF Products)

Synonyms: VWF antibody, si:ch1073-474e24.1 antibody, F8VWF antibody, VWD antibody, 6820430P06Rik antibody, AI551257 antibody, B130011O06Rik antibody, C630030D09 antibody, von Willebrand factor antibody, Von Willebrand factor antibody, VWF antibody, vwf antibody, Vwf antibody

Background: Von Willebrand factor (VWF) is a blood glycoprotein involved in hemostasis. It is mapped to 12p13.31. The VWF gene encodes von Willebrand factor (VWF), a large multimeric glycoprotein that plays a central role in the blood coagulation system, serving both as a major mediator of platelet-vessel wall interaction and platelet adhesion, and as a carrier for coagulation factor VIII. VWF released from endothelial cell Weibel-Palade bodies bound particularly avidly to the extracellular matrix. VWF deficiency or dysfunction (von Willebrand disease) leads to a bleeding tendency, which is most apparent in tissues having high blood flow shear in narrow vessels.

Synonyms: Coagulation factor VIII antibody|Coagulation factor VIII VWF antibody|F8VWF antibody|Factor VIII related antigen antibody|von Willebrand antigen 2 antibody|Von Willebrand antigen II antibody|Von Willebrand disease antibody|VWD antibody|VWF antibody|VWF_HUMAN antibody

Gene ID: 7450

UniProt: P04275