COL4a2 antibody (C-Term)

Catalog No. ABIN3042317

The Rabbit Polyclonal anti-COL4a2 antibody has been validated for WB and IHC. It is suitable to detect COL4a2 in samples from Human. There are 13+ publications available.

Quick Overview for COL4a2 antibody (C-Term) (ABIN3042317)

Target: COL4a2 (COL4A2) (Collagen, Type IV, alpha 2 (COL4A2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This COL4a2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-COL4a2 Antibody

Binding Specificity: AA 1697-1712, C-Term

Purpose: Anti-Canstatin Antibody Picoband®

Sequence: LIRTHISRCQ VCMKNL

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-Canstatin Antibody (ABIN3042317). Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human Canstatin, identical to the related rat and mouse sequences.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human
1. Hwang-Bo J, Hyun Yoo K, Park JH, Jeong HS, Chung IS.:Recombinant canstatin inhibits angiopoietin-1-induced angiogenesis and lymphangiogenesis. Int J Cancer. 2011 Aug 5. 2. Hwang-Bo J, Yoo KH, Jeong HS, Chung IS.Recombinant canstatin inhibits tumor growth in an orthotopic AT-84 oral squamous cell carcinoma model. Biotechnol Lett. 2010 Feb,32(2):189-94. Epub 2009 Oct 17.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P), IHC(F) and ICC.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

References for anti-COL4a2 antibody (ABIN3042317)

Zhu, Chen, Chen, Yang, Xie: "Astaxanthin Promotes Nrf2/ARE Signaling to Alleviate Renal Fibronectin and Collagen IV Accumulation in Diabetic Rats." in: Journal of diabetes research, Vol. 2018, pp. 6730315, (2018) (PubMed).

Luo, Deng, Lamsal, Xu, Xiang, Cheng: "AMP-activated protein kinase alleviates extracellular matrix accumulation in high glucose-induced renal fibroblasts through mTOR signaling pathway." in: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, Vol. 35, Issue 1, pp. 191-200, (2015) (PubMed).

Qiu, Zhang, Zhao, Zhang, Wu, Guo, Hu: "Enhancement of endothelial differentiation of adipose derived mesenchymal stem cells by a three-dimensional culture system of microwell." in: Biomaterials, Vol. 53, pp. 600-8, (2015) (PubMed).

Liu, Lu, Li, Wei, Li: "Engineering blood vessels through micropatterned co-culture of vascular endothelial and smooth muscle cells on bilayered electrospun fibrous mats with pDNA inoculation." in: Acta biomaterialia, Vol. 11, pp. 114-25, (2014) (PubMed).

Ji, Wu, Ma, Ma, Qin: "The effect of resveratrol on the expression of AdipoR1 in kidneys of diabetic nephropathy." in: Molecular biology reports, Vol. 41, Issue 4, pp. 2151-9, (2014) (PubMed).

Wenbin, Guojun: "Resveratrol Ameliorates Diabetes-induced Renal Damage through Regulating the Expression of TGF-?1, Collagen IV and Th17/Treg-related Cytokines in Rats." in: The West Indian medical journal, Vol. 63, Issue 1, pp. 20-5, (2014) (PubMed).

Chen, Wan, Xia, Guo, Wang, Liu, Li: "Promoted regeneration of mature blood vessels by electrospun fibers with loaded multiple pDNA-calcium phosphate nanoparticles." in: European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fu?r Pharmazeutische Verfahrenstechnik e.V, Vol. 85, Issue 3 Pt A, pp. 699-710, (2013) (PubMed).

Xu, Zheng, He, Li, Zhang, Liang: "Puerarin, isolated from Pueraria lobata (Willd.), protects against hepatotoxicity via specific inhibition of the TGF-?1/Smad signaling pathway, thereby leading to anti-fibrotic effect." in: Phytomedicine : international journal of phytotherapy and phytopharmacology, Vol. 20, Issue 13, pp. 1172-9, (2013) (PubMed).

Wen, Huang, Zhang, Zhang, Chen, Gu, Hao: "Resveratrol attenuates diabetic nephropathy via modulating angiogenesis." in: PLoS ONE, Vol. 8, Issue 12, pp. e82336, (2013) (PubMed).

He, Xia, Wang, Wei, Luo, Li: "Multiple release of polyplexes of plasmids VEGF and bFGF from electrospun fibrous scaffolds towards regeneration of mature blood vessels." in: Acta biomaterialia, Vol. 8, Issue 7, pp. 2659-69, (2012) (PubMed).

Qi, Kai-Chen, Liu, Su, Yu: "Protective effect of Icariin on the early stage of experimental diabetic nephropathy induced by streptozotocin via modulating transforming growth factor ?1 and type IV collagen expression in rats." in: Journal of ethnopharmacology, Vol. 138, Issue 3, pp. 731-6, (2011) (PubMed).

Zhang, Wu, Zheng, Li: "Adenovirus-mediated decorin gene transfection has therapeutic effects in a streptozocin-induced diabetic rat model." in: Nephron. Experimental nephrology, Vol. 116, Issue 1, pp. e11-21, (2010) (PubMed).

Xue, Zhang, Liu, Wang: "A new ATP-sensitive potassium channel opener protects the kidney from hypertensive damage in spontaneously hypertensive rats." in: The Journal of pharmacology and experimental therapeutics, Vol. 315, Issue 2, pp. 501-9, (2005) (PubMed).

Target Details for COL4a2

Target: COL4a2 (COL4A2) (Collagen, Type IV, alpha 2 (COL4A2))

Alternative Name: COL4A2

Background:

Synonyms: Collagen alpha-2 (IV) chain,Canstatin,COL4A2,

Tissue Specificity: Widely expressed. Differentially expressed in various brain regions. .

Background: Canstatin, a cleavage product corresponding to the collagen alpha 2 (IV) NC1 domain, possesses both anti-angiogenic and anti-tumor cell activity. It inhibits proliferation and migration of endothelial cells, reduces mitochondrial membrane potential, and induces apoptosis. Specifically induces Fas-dependent apoptosis and activates procaspase-8 and -9 activity. Ligand for alphavbeta3 and alphavbeta5 integrins.

Sequence Similarities: Belongs to the type IV collagen family.

Molecular Weight: 56 kDa

UniProt: P08572