Splicing Factor 1 antibody (N-Term)

Catalog No. ABIN3032719

Product Details anti-Splicing Factor 1 Antibody

Target: Splicing Factor 1 (SF1)

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Splicing Factor 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Purification: Antigen affinity

Immunogen: An amino acid sequence from the N-terminus of human Splicing factor 1 (DFPSKKRKRSRWNQDTMEQK) was used as the immunogen for this Splicing factor 1 antibody (100% homologous in human, mouse and rat).

Isotype: IgG

Application Details

Application Notes: The stated application concentrations are suggested starting amounts. Titration of the Splicing factor 1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL,IHC (Paraffin): 0.5-1 μg/mL,IHC (Frozen): 0.5-1 μg/mL,Immunocytochemistry: 0.5-1 μg/mL

Restrictions: For Research Use only

Handling

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: -20 °C

Storage Comment: After reconstitution, the Splicing factor 1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Target Details for Splicing Factor 1

Target: Splicing Factor 1 (SF1)

Alternative Name: Splicing Factor 1 (SF1 Products)

Background: Splicing factor 1, also known a sSF1, Zinc finger protein 162 (ZNF162), ZFM162, Transcription factor ZFM1, and Mammalian branch point-binding protein (BBP or mBBP), is a protein that in humans is encoded by the SF1 gene. It ispredicted 623-amino acid protein. RT-PCR was used to show expression in the thyroid gland, pancreas, adrenal gland, and ovary. All of the isoforms contain a KH domain, a sequence motif present in proteins playinig a major role in regulating cellular RNA metabolism.

UniProt: Q15637

Pathways: Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Ribonucleoprotein Complex Subunit Organization, Maintenance of Protein Location