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SPARC antibody (AA 284-303)

SPARC Reactivity: Human WB, IHC (p) Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN3032523
  • Target See all SPARC Antibodies
    SPARC (Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC))
    Binding Specificity
    • 15
    • 10
    • 8
    • 7
    • 5
    • 5
    • 4
    • 4
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 284-303
    Reactivity
    • 85
    • 40
    • 35
    • 10
    • 6
    • 5
    • 4
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Host
    • 78
    • 20
    • 4
    • 2
    • 2
    Rabbit
    Clonality
    • 82
    • 24
    Polyclonal
    Conjugate
    • 59
    • 11
    • 8
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This SPARC antibody is un-conjugated
    Application
    • 85
    • 39
    • 29
    • 22
    • 13
    • 13
    • 10
    • 10
    • 10
    • 9
    • 8
    • 5
    • 5
    • 5
    • 5
    • 3
    Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Purification
    Antigen affinity
    Immunogen
    Amino acids 284-303 (DEWAGCFGIKQKDIDKDLVI-human) were used as the immunogen for this SPARC antibody.
    Isotype
    IgG
    Top Product
    Discover our top product SPARC Primary Antibody
  • Application Notes
    The stated application concentrations are suggested starting amounts. Titration of the SPARC antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL,IHC (Paraffin): 0.5-1 μg/mL
    Restrictions
    For Research Use only
  • Buffer
    0.5 mg/mL if reconstituted with 0.2 mL sterile DI water
    Storage
    -20 °C
    Storage Comment
    After reconstitution, the SPARC antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
  • Target
    SPARC (Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC))
    Alternative Name
    SPARC (SPARC Products)
    Synonyms
    ON antibody, AA517111 antibody, BM-40 antibody, hm:zeh0062 antibody, SPARC antibody, sparc antibody, secreted protein acidic and cysteine rich antibody, secreted acidic cysteine rich glycoprotein antibody, secreted protein, acidic, cysteine-rich (osteonectin) antibody, secreted protein, acidic, cysteine-rich (osteonectin) L homeolog antibody, secreted protein, acidic, cysteine-rich (osteonectin) S homeolog antibody, SPARC antibody, Sparc antibody, sparc antibody, sparc.L antibody, sparc.S antibody
    Background
    Secreted protein acidic and rich in cysteine, also known as Osteonectin, is a protein that in humans is encoded by the SPARC gene. The human gene is 26.5 kb long, and contains 10 exons and 9 introns and is located on chromosome 5q31-q33. SPARC is an acidic, cysteine-rich glycoprotein consisting of a single polypeptide chain that can be broken into 4 domains: 1) an Ca++ binding domains near the glutamic acidic-rich region at the amino terminus(domain I), 2) a cysteine- rich(domain II), 3) a hydrophilic region(domain III) and 4) an EF hand motif at the carboxy terminus region(domain IV). SPARC is a glycoprotein in the bone that binds sodium. It is secreted by osteoblasts during bone formation, initiating mineralization and promoting mineral crystal formation. It also shows affinity for collagen in addition to bone mineral calcium. A correlation between SPARC over expression and ampullary cancers and chronic pancreatitis has been found.
    UniProt
    P09486
    Pathways
    Autophagy
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