PDK2 antibody (C-Term)

Catalog No. ABIN3032416

Product Details anti-PDK2 Antibody

Target: PDK2 (Pyruvate Dehydrogenase Kinase, Isozyme 2 (PDK2))

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PDK2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Purification: Antigen affinity

Immunogen: An amino acid sequence from the C-terminus of human PDK2 (WRHYQTIQEAGDWC) was used as the immunogen for this PDK2 antibody (100% homologous in human, mouse and rat).

Isotype: IgG

Application Details

Application Notes: The stated application concentrations are suggested starting amounts. Titration of the PDK2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL,IHC (Paraffin): 0.5-1 μg/mL,Immunocytochemistry: 0.5-1 μg/mL

Restrictions: For Research Use only

Handling

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: -20 °C

Storage Comment: After reconstitution, the PDK2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Target Details for PDK2

Target: PDK2 (Pyruvate Dehydrogenase Kinase, Isozyme 2 (PDK2))

Alternative Name: PDK2 (PDK2 Products)

Synonyms: PDHK2 antibody, PDKII antibody, Pdk antibody, zgc:56209 antibody, PDK2 antibody, pyruvate dehydrogenase kinase 2 antibody, pyruvate dehydrogenase kinase, isoenzyme 2 antibody, pyruvate dehydrogenase kinase, isozyme 2b antibody, PDK2 antibody, Pdk2 antibody, pdk2b antibody

Background: Pyruvate dehydrogenase kinase isoenzyme 2, is an enzyme that in humans is encoded by the PDK2 gene. To find human PDKs, Gudi et al.(1995) used oligonucleotide primers to screen a human liver cDNA library by PCR. The human PDK2 gene encodes a protein with a predicted molecular mass of 46,181 Da that shares 96 % identity with that of the rat gene. Northern blot analysis showed that the PDK2 message was found in many tissues, with the highest level in heart and skeletal muscle, intermediate levels in brain, kidney, pancreas and liver, and low levels in placenta and lung.

UniProt: Q15119

Pathways: PI3K-Akt Signaling, RTK Signaling, Carbohydrate Homeostasis, Regulation of Carbohydrate Metabolic Process, Warburg Effect