PTTG1 antibody (AA 146-161)

Catalog No. ABIN3032234

Product Details anti-PTTG1 Antibody

Target: PTTG1 (Pituitary Tumor-Transforming 1 (PTTG1))

Binding Specificity: AA 146-161

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PTTG1 antibody is un-conjugated

Application: Western Blotting (WB)

Purification: Antigen affinity

Immunogen: Amino acids 146-161 (LMILDEERELEKLFQL-human) were used as the immunogen for this Securin antibody.

Isotype: IgG

Application Details

Application Notes: The stated application concentrations are suggested starting amounts. Titration of the Securin antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL

Restrictions: For Research Use only

Handling

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: -20 °C

Storage Comment: After reconstitution, the Securin antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Target Details for PTTG1

Target: PTTG1 (Pituitary Tumor-Transforming 1 (PTTG1))

Alternative Name: Securin (PTTG1 Products)

Background: Securin, also known as pituitary tumor-transforming 1 or EAP1 is a protein that in humans is encoded by the PTTG1 gene. The encoded protein is a homolog of yeast securin proteins, which prevent separins from promoting sister chromatid separation. It is an anaphase-promoting complex(APC) substrate that associates with a separin until activation of the APC. The gene product has transforming activity in vitro and tumorigenic activity in vivo, and is highly expressed in various tumors. Securin contains 2 PXXP motifs, which are required for its transforming and tumorigenic activities, as well as for its stimulation of basic fibroblast growth factor expression. It also contains a destruction box(D box) that is required for its degradation by the APC. The acidic C-terminal region of the encoded protein can act as a transactivation domain. It is mainly a cytosolic protein, although it partially localizes in the nucleus.

UniProt: O95997