MPG antibody (C-Term)

Catalog No. ABIN3031925

Product Details anti-MPG Antibody

Target: MPG (N-Methylpurine-DNA Glycosylase (MPG))

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MPG antibody is un-conjugated

Application: Western Blotting (WB)

Purification: Antigen affinity

Immunogen: An amino acid sequence from the C-terminus of human MPG (NKSFDQRDLAQDEAVW) was used as the immunogen for this MPG antibody.

Isotype: IgG

Application Details

Application Notes: The stated application concentrations are suggested starting amounts. Titration of the MPG antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL

Restrictions: For Research Use only

Handling

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: -20 °C

Storage Comment: After reconstitution, the MPG antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Target Details for MPG

Target: MPG (N-Methylpurine-DNA Glycosylase (MPG))

Alternative Name: MPG (MPG Products)

Background: N-Methylpurine-DNA glycosylase is encoded by the MPG gene, mapped to human chromosome 16 by analysis of a panel of DNAs from mouse/human and hamster/human hybrid cell lines. The gene was expressed in all cell lines and tissues examined, but was found at particularly high levels in a colon adenocarcinoma cell line (HT29). The completely characterized human gene was found to span 7 to 8 kb of genomic DNA and to be localized 75 kb upstream of the embryonic zeta-globin gene. To assess the contribution of MPG to the repair of several mutagenic lesions in vivo, Hang et al.(1997) biochemically analyzed cell-free extracts of tissues from mice with a targeted deletion of the gene. Following treatment with DNA-methylating agents, increased persistence of 7-methylguanine (meG) was found in liver sections of MPG knockout mice in comparison with wildtype mice, demonstrating an in vivo phenotype for the MPG-null animals.

UniProt: P29372

Pathways: DNA Damage Repair