Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

Myosin Binding Subunit antibody (N-Term)

MBS Reactivity: Human, Mouse, Rat WB, FACS Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN3031917
  • Target See all Myosin Binding Subunit (MBS) products
    Myosin Binding Subunit (MBS)
    Binding Specificity
    N-Term
    Reactivity
    Human, Mouse, Rat
    Host
    • 1
    Rabbit
    Clonality
    • 1
    Polyclonal
    Conjugate
    • 1
    This Myosin Binding Subunit antibody is un-conjugated
    Application
    Western Blotting (WB), Flow Cytometry (FACS)
    Purification
    Antigen affinity
    Immunogen
    An amino acid sequence from the N-terminus of human Myosin Phosphatase (MKMADAKQKRNEQLKRW) was used as the immunogen for this Myosin Phosphatase antibody (100% homologous in human, mouse and rat).
    Isotype
    IgG
  • Application Notes
    The stated application concentrations are suggested starting amounts. Titration of the Myosin Phosphatase antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL, FACS: 1-3 μg/10^6 cells
    Restrictions
    For Research Use only
  • Buffer
    0.5 mg/mL if reconstituted with 0.2 mL sterile DI water
    Storage
    -20 °C
    Storage Comment
    After reconstitution, the Myosin Phosphatase antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
  • Target
    Myosin Binding Subunit (MBS)
    Alternative Name
    Myosin Phosphatase (MBS Products)
    Background
    PPP1R12A, also called MYPT1 (Myosin phosphatase target subunit 1), is an enzyme that in humans is encoded by the PPP1R12A gene. Sequencing analysis showed that human PPP1R12A contains 1,030 amino acids with a calculated molecular mass of approximately 115 kD. PPP1R12A is the protein that regulates PP1 function in smooth muscle relaxation. The cellular MYPT1-PP1-delta -specific inhibitor CPI17 caused a loss of merlin function characterized by merlin phosphorylation, Ras activation, and transformation. Jin et al. concluded that PPP1R12A and its substrate merlin are part of a previously undescribed tumor suppressor cascade that can be hindered in 2 ways, by mutation of the NF2 gene and by upregulation of the oncoprotein CPI17.
    UniProt
    O14974
You are here:
Support