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MBD4 antibody (C-Term)

MBD4 Reactivity: Human, Mouse, Rat WB Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN3031772
  • Target See all MBD4 Antibodies
    MBD4 (Methyl-CpG Binding Domain Protein 4 (MBD4))
    Binding Specificity
    • 9
    • 8
    • 8
    • 6
    • 6
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    C-Term
    Reactivity
    • 32
    • 4
    • 2
    • 1
    Human, Mouse, Rat
    Host
    • 31
    • 1
    • 1
    Rabbit
    Clonality
    • 32
    • 1
    Polyclonal
    Conjugate
    • 16
    • 4
    • 4
    • 3
    • 3
    • 3
    This MBD4 antibody is un-conjugated
    Application
    • 26
    • 24
    • 10
    • 2
    • 2
    • 1
    • 1
    • 1
    Western Blotting (WB)
    Purification
    Antigen affinity
    Immunogen
    An amino acid sequence from the C-terminus of human Methyl-CpG-Binding Domain Protein 4 (YHDWLWENHEKLSLS) was used as the immunogen for this MBD4 antibody (100% homologous in human, mouse and rat).
    Isotype
    IgG
    Top Product
    Discover our top product MBD4 Primary Antibody
  • Application Notes
    The stated application concentrations are suggested starting amounts. Titration of the MBD4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL
    Restrictions
    For Research Use only
  • Buffer
    0.5 mg/mL if reconstituted with 0.2 mL sterile DI water
    Storage
    -20 °C
    Storage Comment
    After reconstitution, the MBD4 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
  • Target
    MBD4 (Methyl-CpG Binding Domain Protein 4 (MBD4))
    Alternative Name
    MBD4 (MBD4 Products)
    Synonyms
    MED1 antibody, Med1 antibody, methyl-CpG binding domain 4, DNA glycosylase antibody, methyl-CpG binding domain protein 4 antibody, methyl-CpG binding domain 4 DNA glycosylase antibody, MBD4 antibody, Mbd4 antibody
    Background
    Methyl-CpG-Binding Domain Protein 4, also known as MED1, is a protein that in humans is encoded by the MBD4 gene. MBD4 specifically binds methylated DNA, colocalizes with methylated sequences, and is likely to mediate the effects of DNA methylation in mammalian cells(Hendrich and Bird, 1998). Riccio et al.(1999) mapped the gene to chromosome 3q21-q22 by FISH. Hendrich and Bird(1998) found that both MBD2 and -4 specifically bound methylated DNA in vitro and colocalized with methylated sequences in vivo. They concluded that MBD2 and -4 are likely to be mediators of the effects of DNA methylation in mammalian cells. Hendrich et al.(1999) showed that MBD4 contains a methyl-CpG-binding domain that can efficiently remove thymine or uracil from mismatched CpG sites in vitro. Furthermore, the methyl-CpG-binding domain of MBD4 binds preferentially to 5-methylcytosine CpG-TpG mismatches--the primary product of deamination at methyl-CpG.
    UniProt
    O95243
    Pathways
    DNA Damage Repair
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