IBSP antibody (N-Term)

Catalog No. ABIN3031383

Product Details anti-IBSP Antibody

Target: IBSP (Integrin-Binding Sialoprotein (IBSP))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IBSP antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purification: Antigen affinity

Immunogen: An amino acid sequence from the N-terminus of human Bone Sialoprotein 2 (FSMKNLHRRVKIEDSEEN) was used as the immunogen for this IBSP antibody.

Isotype: IgG

Application Details

Application Notes: The stated application concentrations are suggested starting amounts. Titration of the IBSP antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL,IHC (Paraffin): 0.5-1 μg/mL

Restrictions: For Research Use only

Handling

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: -20 °C

Storage Comment: After reconstitution, the IBSP antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Target Details for IBSP

Target: IBSP (Integrin-Binding Sialoprotein (IBSP))

Alternative Name: IBSP / Bone Sialoprotein 2 (IBSP Products)

Synonyms: BNSP antibody, BSP antibody, BSP-II antibody, SP-II antibody, Bsp antibody, BSP II antibody, BSPII antibody, Bsp2 antibody, IBSP antibody, integrin binding sialoprotein antibody, integrin-binding sialoprotein antibody, integrin-binding sialoprotein S homeolog antibody, IBSP antibody, Ibsp antibody, ibsp.S antibody, ibsp antibody

Background: Integrin-binding sialoprotein, also known as Bone sialoprotein and BSP, is a major structural protein of the bone matrix. Bone sialoprotein is a predicted 35KD acidic glycoprotein that undergoes extensive posttranslational modifications and is visualized by western blot at up to 70KD. It constitutes approximately 12 % of the noncollagenous proteins in human bone and is synthesized by skeletal-associated cell types, including hypertrophic chondrocytes, osteoblasts, osteocytes, and osteoclasts. The only extraskeletal site of its synthesis is the trophoblast. This protein binds to calcium and hydroxyapatite via its acidic amino acid clusters, and mediates cell attachment through an RGD sequence that recognizes the vitronectin receptor. The BSP gene is mapped to 4q22.1.

UniProt: P21815