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GLI3 antibody (N-Term)

GLI3 Reactivity: Human, Mouse, Rat WB Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN3031041
  • Target See all GLI3 Antibodies
    GLI3 (GLI Family Zinc Finger 3 (GLI3))
    Binding Specificity
    • 7
    • 5
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    N-Term
    Reactivity
    • 44
    • 23
    • 16
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human, Mouse, Rat
    Host
    • 37
    • 5
    • 3
    Rabbit
    Clonality
    • 33
    • 12
    Polyclonal
    Conjugate
    • 24
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    This GLI3 antibody is un-conjugated
    Application
    • 17
    • 13
    • 7
    • 6
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB)
    Purification
    Antigen affinity
    Immunogen
    An amino acid sequence from the N-terminus of human Gli-kruppel family member 3 (SNEDESPGQTYHRERRNA) was used as the immunogen for this Gli3 antibody.
    Isotype
    IgG
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    Discover our top product GLI3 Primary Antibody
  • Application Notes
    The stated application concentrations are suggested starting amounts. Titration of the Gli3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
    1. There are two isoforms of Gli-kruppel family member 3: the 170-190KD full length form and a truncated ~80KD form. This antibody will detect both, depending on the testing material.\. Western blot: 0.5-1 μg/mL (1)
    Restrictions
    For Research Use only
  • Buffer
    0.5 mg/mL if reconstituted with 0.2 mL sterile DI water
    Storage
    -20 °C
    Storage Comment
    After reconstitution, the Gli3 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
  • Target
    GLI3 (GLI Family Zinc Finger 3 (GLI3))
    Alternative Name
    Gli3 (GLI3 Products)
    Synonyms
    ACLS antibody, GCPS antibody, GLI3-190 antibody, GLI3FL antibody, PAP-A antibody, PAPA antibody, PAPA1 antibody, PAPB antibody, PHS antibody, PPDIV antibody, xgli3 antibody, AI854843 antibody, AU023367 antibody, Bph antibody, Pdn antibody, Xt antibody, add antibody, im:7142603 antibody, GLI3 antibody, GLI family zinc finger 3 antibody, GLI family zinc finger 3 L homeolog antibody, GLI-Kruppel family member GLI3 antibody, GLI3 antibody, Gli3 antibody, gli3.L antibody, gli3 antibody
    Background
    Gli-kruppel family member 3 is a member of the zinc finger family related to Kruppel, a protein family that is known to regulate development in Drosophila. The gene is expressed as an 8.5-kb mRNA in tissues such as testis, myometrium, placenta, and lung, and the protein product (relative molecular mass, 190,000D) shows sequence-specific DNA binding. Gli3 is homologous to the Drosophila cubitus interruptus (ci) gene product(Ci), which regulates the Patched (pct), Gooseberry (gsb), and Decapentaplegic (dpp) genes. PKA-dependent processing of vertebrate Gli3 in developing limb generates a potent repressor in a manner antagonized by apparent long-range signaling from posteriorly localized Sonic hedgehog protein. The high relative abundance and potency of Gli3 repressor suggested specialization of the protein and its products for negative hedgehog pathway regulation.
    UniProt
    P10071
    Pathways
    Hedgehog Signaling
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