Flotillin 2 antibody (N-Term)

Catalog No. ABIN3030973

Product Details anti-Flotillin 2 Antibody

Target: Flotillin 2 (FLOT2)

Binding Specificity: N-Term

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Flotillin 2 antibody is un-conjugated

Application: Western Blotting (WB)

Purification: Antigen affinity

Immunogen: An amino acid sequence from the N-terminus of human FLOT2 (TLTVEQIYQDRDQFA) was used as the immunogen for this Flotillin 2 antibody (100% homologous in human, mouse and rat).

Isotype: IgG

Application Details

Application Notes: The stated application concentrations are suggested starting amounts. Titration of the Flotillin 2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL

Restrictions: For Research Use only

Handling

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: -20 °C

Storage Comment: After reconstitution, the Flotillin 2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Target Details for Flotillin 2

Target: Flotillin 2 (FLOT2)

Alternative Name: Flotillin 2 (FLOT2 Products)

Synonyms: ECS-1 antibody, ECS1 antibody, ESA antibody, ESA1 antibody, M17S1 antibody, AI573412 antibody, Esa antibody, reggie-2 antibody, CG11547 antibody, CG14409 antibody, CG32593 antibody, DmFlo2 antibody, Dmel\\CG32593 antibody, FLO2 antibody, FLO[Dm]-2 antibody, Flo2 antibody, flo2 antibody, reggie-1 antibody, ecs-1 antibody, ecs1 antibody, esa antibody, esa1 antibody, flotillin-2 antibody, FLOT2 antibody, fb48a04 antibody, im:7143869 antibody, wu:fb48a04 antibody, zgc:55821 antibody, flotillin 2 antibody, Flotillin 2 antibody, flotillin 2 L homeolog antibody, flotillin 2a antibody, FLOT2 antibody, Flot2 antibody, Flo2 antibody, flot2.L antibody, flot2 antibody, flot2a antibody

Background: Flotillin 2, also known as ESA1 or M17S1, is a protein that in humans is encoded by the FLOT2 gene. Schroeder et al.(1991) isolated a cDNA for an epidermal surface antigen believed to be involved in epidermal cell adhesion. By analysis of a somatic cell hybrid panel and in situ hybridization using the FLOT2 cDNA, the gene was mapped to 17q11-q12 in the region containing the NF1 gene. Bickel et al.(1997) found that mouse Flot2 consistently copurifies with Flot1 and with caveolin-1 in the purification of caveolin-rich membranes. Using a quantitative proteomic analysis of cultured mouse neuronal stem cells, Li et al.(2012) found that palmitoylation and oligomerization of Flotillin2 was abolished in homozygous Dhhc5 mutant neuronal stem cells. The absolute amount of Flotillin2 was not changed in Dhhc5 mutant neurons.

UniProt: Q14254