An amino acid sequence from the C-terminus of human Eukaryotic Translation Initiation Factor 3, Subunit B (EDFRKYRKMAQELYMEQKNER) was used as the immunogen for this Eif3b antibody.
The stated application concentrations are suggested starting amounts. Titration of the EIF3B antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL
Restrictions
For Research Use only
Buffer
0.5 mg/mL if reconstituted with 0.2 mL sterile DI water
Storage
-20 °C
Storage Comment
After reconstitution, the EIF3B antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Target
EIF3B
(Eukaryotic Translation Initiation Factor 3, Subunit B (EIF3B))
Eukaryotic Translation Initiation Factor 3, Subunit B, also called PRT1 or EIF3-p116, is a protein that in humans is encoded by the EIF3B gene. By searching an EST database, Methot et al.(1997) identified a cDNA encoding a human Prt1 homolog. Asano et al.(1997) demonstrated that the 115-kD component of HeLa cell eIF3 is actually composed of two proteins, p116 (EIF3B) and an unrelated 110-kD protein.