An amino acid sequence from the C-terminus of human EIF2 beta (FQAVTGKRAQLRAKAN) was used as the immunogen for this EIF2B antibody (100% homologous in human, mouse and rat).
EIF2S2
Reactivity: Human, Rat
WB
Host: Rabbit
Polyclonal
unconjugated
Application Notes
The stated application concentrations are suggested starting amounts. Titration of the EIF2B antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL
Restrictions
For Research Use only
Buffer
0.5 mg/mL if reconstituted with 0.2 mL sterile DI water
Storage
-20 °C
Storage Comment
After reconstitution, the EIF2B antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Eukaryotic translation initiation factor 2 subunit 2, also called EIF2B, is a protein that in humans is encoded by the EIF2S2 gene. This gene belongs to the EIF-2-beta family. Eukaryotic translation initiation factor 2 (EIF2) functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA and binding to a 40S ribosomal subunit. EIF2 is composed of three subunits, alpha, beta, and gamma, with the protein encoded by this gene representing the beta subunit. The beta subunit catalyzes the exchange of GDP for GTP, which recycles the EIF2 complex for another round of initiation.