The stated application concentrations are suggested starting amounts. Titration of the CD3 epsilon antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL,IHC (Paraffin): 0.5-1 μg/mL,IHC (Frozen): 0.5-1 μg/mL,Immunocytochemistry: 0.5-1 μg/mL
Restrictions
For Research Use only
Buffer
0.5 mg/mL if reconstituted with 0.2 mL sterile DI water
Storage
-20 °C
Storage Comment
After reconstitution, the CD3 epsilon antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
CD3 epsilon is a polypeptide which in humans is encoded by the CD3E gene which resides on chromosome 11. It is mapped to 11q23.3. Together with CD3gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, CD3e forms the T cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the < font face=symbol>e, < font face=symbol>g and < font face=symbol>d polypeptides are located in the same cluster on chromosome 11. The < font face=symbol>e-polypeptide plays an essential role in T-cell development.