Caspase 10 antibody (AA 220-236)

Catalog No. ABIN3030289

Product Details anti-Caspase 10 Antibody

Target: Caspase 10 (CASP10) (Caspase 10, Apoptosis-Related Cysteine Peptidase (CASP10))

Binding Specificity: AA 220-236

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Caspase 10 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purification: Antigen affinity

Immunogen: Amino acids 220-236 (VKTFLEALPRAAVYRMN-human) were used as the immunogen for this Caspase-10 antibody. This sequence is from the large subunit

Isotype: IgG

Application Details

Application Notes: The stated application concentrations are suggested starting points. Titration of the Caspase-10 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL,IHC (Paraffin): 0.5-1 μg/mL

Restrictions: For Research Use only

Handling

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: -20 °C

Storage Comment: After reconstitution, the Caspase-10 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Target Details for Caspase 10

Target: Caspase 10 (CASP10) (Caspase 10, Apoptosis-Related Cysteine Peptidase (CASP10))

Alternative Name: Caspase-10 (CASP10 Products)

Synonyms: xCaspase-10 antibody, alps2 antibody, caspace-10 antibody, caspase-10 antibody, flice2 antibody, mch4 antibody, xCaspace-10 antibody, ALPS2 antibody, FLICE2 antibody, MCH4 antibody, caspase 10 S homeolog antibody, caspase 10 antibody, caspase-10 antibody, casp10.S antibody, casp10 antibody, CASP10 antibody, Casp10 antibody, LOC100848685 antibody

Background: Caspase-10 is an enzyme that in humans is encoded by the CASP10 gene. The CASP10 gene contains 11 exons and spans about 48 kb. This gene is mapped to 2q33.1. It is transcribed in the centromere-to-telomere direction. This gene encodes a protein that is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes that undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates CASP3 and 7, and the protein itself is processed by CASP8. Mutations in this gene are associated with apoptosis defects seen in type II autoimmune lymphoproliferative syndrome. Three alternatively spliced transcript variants encoding different isoforms have been described for this protein.

UniProt: Q92851

Pathways: Apoptosis, Caspase Cascade in Apoptosis