Calpain antibody (Catalytic Subunit)
Reactivity: Human, Mouse, Rat
WB, IHC (p)
Host: Rabbit
Polyclonal
unconjugated
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- Target See all Calpain products
- Calpain
- Binding Specificity
- AA 396-555, Catalytic Subunit
- Reactivity
- Human, Mouse, Rat
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This Calpain antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Purification
- Antigen affinity
- Immunogen
- Human recombinant protein from the CAPN1 catalytic subunit (AA 396-555) was used as the immunogen for this Calpain antibody.
- Isotype
- IgG
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- Application Notes
- The stated application concentrations are suggested starting amounts. Titration of the Calpain antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL,IHC (Paraffin): 0.5-1 μg/mL
- Restrictions
- For Research Use only
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- Buffer
- 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water
- Storage
- -20 °C
- Storage Comment
- After reconstitution, the Calpain antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
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- Target
- Calpain
- Abstract
- Calpain Products
- Synonyms
- CG18152 antibody, CG7563 antibody, Dmel\\CG7563 antibody, EST C antibody, calpain antibody, Tb08.28A12.350 antibody, Calpain-A antibody, hypothetical protein antibody, calpain 11 antibody, calpain antibody, CalpA antibody, T11A5.6 antibody, CAPN11 antibody, Tb927.8.8330 antibody
- Background
- Calpain 1 is also known as CAPN, CAPN1 and muCL. The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. The CPN1 gene encodes the catalytic subunit of the ubiquitous enzyme, Calpain 1. Several transcript variants encoding two different isoforms have been found for this gene.
- Gene ID
- 823
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