The stated application concentrations are suggested starting amounts. Titration of the AHR antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL,IHC (Paraffin): 0.5-1 μg/mL
Restrictions
For Research Use only
Buffer
0.5 mg/mL if reconstituted with 0.2 mL sterile DI water
Storage
-20 °C
Storage Comment
After reconstitution, the AHR antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Aryl hydrocarbon receptor is a member of the family of basic helix-loop-helix transcription factors. It is a cytosolic transcription factor that is normally inactive, bound to several co-chaperones. Estrogenic actions of AHR agonists were detected in wildtype ovariectomized mouse uteri, but were absent in Ahr -/- or Er-alpha -/- ovariectomized mice. Complex assembly and ubiquitin ligase activity of CUL4B/AHR in vitro and in vivo are dependent on the AHR ligand. In the CUL4B/AHR complex, ligand-activated AHR acts as a substrate-specific adaptor component that targets sex steroid receptors for degradation.