This Angiotensin I Converting Enzyme 1 antibody is un-conjugated
Application
Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Purification
Antigen affinity
Immunogen
An amino acid sequence from the middle region of human Angiotensin Converting Enzyme 1 (TQARKFDVNQLQNT) was used as the immunogen for this ACE antibody.
The stated application concentrations are suggested starting amounts. Titration of the ACE1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL,IHC (Paraffin): 0.5-1 μg/mL
Restrictions
For Research Use only
Buffer
0.5 mg/mL if reconstituted with 0.2 mL sterile DI water
Storage
-20 °C
Storage Comment
After reconstitution, the ACE antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Target
Angiotensin I Converting Enzyme 1 (ACE)
(Angiotensin I Converting Enzyme (Peptidyl-Dipeptidase A) 1 (ACE))
Angiotensin converting enzyme, also called DCP or CD143, is a zinc-containing dipeptidyl carboxypeptidase widely distributed in mammalian tissues and is though to play a critical role in blood pressure regulation. The ACE gene is mapped to 17q23.3. This gene encodes an enzyme involved in catalyzing the conversion of angiotensin I into a physiologically active peptide angiotensin II. Angiotensin II is a potent vasopressor and aldosterone-stimulating peptide that controls blood pressure and fluid-electrolyte balance. This enzyme plays a key role in the renin-angiotensin system. Many studies have associated the presence or absence of a 287 bp Alu repeat element in this gene with the levels of circulating enzyme or cardiovascular pathophysiologies.