EPH Receptor B3 antibody (C-Term)

Catalog No. ABIN3029711

Product Details anti-EPH Receptor B3 Antibody

Target: EPH Receptor B3 (EPHB3)

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EPH Receptor B3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purification: Antigen affinity

Immunogen: An amino acid sequence from the C-terminus of human Eph receptor B3 (SIQDMRLQMNQTLPVQV) was used as the immunogen for this EphB3 antibody (100% homologous in human, mouse and rat).

Isotype: IgG

Application Details

Application Notes: The stated application concentrations are suggested starting amounts. Titration of the EphB3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL,IHC (Paraffin): 0.5-1 μg/mL

Restrictions: For Research Use only

Handling

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: -20 °C

Storage Comment: After reconstitution, the EphB3 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Target Details for EPH Receptor B3

Target: EPH Receptor B3 (EPHB3)

Alternative Name: EphB3 Ephrin Receptor B3 (EPHB3 Products)

Background: Ephrin Receptor B3, is also known as human embryo kinase2 (HEK2) or Eph-like tyrosine kinase 2 (ETK2) is a member of the EPH/ELK family of tyrosine kinases. The gene encodes a 998-amino acid polypeptide having a single putative transmembrane domain, a secretory signal sequence, and two fibronectin repeats. The gene is mapped to human chromosome 3q21-qter. EphB3 interacts with two ligands of EPH-related kinases (LERKs), LERK2/EFNB1 and LERK5/EFNB2. Coincubation of EphB3- and LERK2-expressing cells induces cell-cell adhesion and aggregation. Additionally, coexpression of the two proteins results in reduced kinase activity of EphB3.

UniProt: P54753

Pathways: RTK Signaling