Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Purification
Antigen affinity
Immunogen
An amino acid sequence from the N-terminus of human Carbonic Anhydrase III (AKEWGYASHNGPDHWHELF) was used as the immunogen for this Carbonic Anhydrase III antibody.
The stated application concentrations are suggested starting amounts. Titration of the CA3 antibody Carbonic Anhydrase III may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL,IHC (Paraffin): 0.5-1 μg/mL
Restrictions
For Research Use only
Buffer
0.5 mg/mL if reconstituted with 0.2 mL sterile DI water
Storage
-20 °C
Storage Comment
After reconstitution, the Carbonic Anhydrase III antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Carbonic anhydrase III (CA3) is an enzyme that in humans is encoded by the CA3 gene. It is a member of a multigene family (at least six separate genes are known) that encode carbonic anhydrase isozymes. The gene spans 10.3 kb and contains seven exons and six introns. The protein is found in high concentration in muscle. It is used in conjunction with creatine kinase detection of the carrier state for Duchenne muscular dystrophy.