CACYBP antibody (Middle Region)

Catalog No. ABIN3029276

Product Details anti-CACYBP Antibody

Target: CACYBP (Calcyclin Binding Protein (CACYBP))

Binding Specificity: Middle Region

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CACYBP antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purification: Antigen affinity

Immunogen: An amino acid sequence from the middle region of human SIAH Interacting Protein (NTRWDYLTQVEKECKE) was used as the immunogen for this SIP antibody (100% homologous in human, mouse and rat).

Isotype: IgG

Application Details

Application Notes: The stated application concentrations are suggested starting amounts. Titration of the SIP antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL,IHC (Paraffin): 0.5-1 μg/mL,Immunocytochemistry: 0.5-1 μg/mL

Restrictions: For Research Use only

Handling

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: -20 °C

Storage Comment: After reconstitution, the SIP antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Target Details for CACYBP

Target: CACYBP (Calcyclin Binding Protein (CACYBP))

Alternative Name: SIP (CACYBP Products)

Synonyms: zgc:76993 antibody, CACYBP antibody, sip antibody, gig5 antibody, pnas-107 antibody, s100a6bp antibody, T1P2.12 antibody, T1P2_12 antibody, GIG5 antibody, RP1-102G20.6 antibody, S100A6BP antibody, SIP antibody, calcyclin binding protein antibody, calcyclin binding protein, putative antibody, Calcyclin binding protein, putative antibody, SGS domain-containing protein antibody, calcyclin binding protein L homeolog antibody, cacybp antibody, CACYBP antibody, PB000926.02.0 antibody, PCHAS_145490 antibody, PVX_100855 antibody, PKH_145680 antibody, AT1G30070 antibody, cacybp.L antibody, Cacybp antibody

Background: CACYBP (Calcyclin-binding protein), also called SIP (SIAH Interacting Protein), is a protein that in humans is encoded by the CACYBP gene. The full-length SIP cDNA encodes a predicted 228-amino acid protein. Sequence analysis of the shortest cDNA derived by 2-hybrid screening revealed an 8-amino acid difference in the deduced open reading frame followed by a stop codon, resulting in a predicted 80-amino acid protein, SIP-short (SIPS). The CACYBP gene is mapped on 1q25.1. It may be involved in calcium-dependent ubiquitination and subsequent proteosomal degradation of target proteins. It probably serves as a molecular bridge in ubiquitin E3 complexes and participates in the ubiquitin-mediated degradation of beta-catenin. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene. The C-terminal region of SIP that is homologous to SGT1 was able to complement defects in yeast strains containing SGT1 mutant alleles, demonstrating conservation of SGT1 and SIP protein function.

UniProt: Q9HB71

Pathways: Response to Growth Hormone Stimulus