TICAM2 antibody (C-Term)

Catalog No. ABIN3029187

Product Details anti-TICAM2 Antibody

Target: TICAM2 (Toll-Like Receptor Adaptor Molecule 2 (TICAM2))

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TICAM2 antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Purification: Antigen affinity

Immunogen: An amino acid sequence from the C-terminus of human Translocating chain-associating membrane protein 1 (KFINFQLRRWREHSAFQA) was used as the immunogen for this TRAM antibody (100% homologous in human, mouse and rat).

Isotype: IgG

Application Details

Application Notes: The stated application concentrations are suggested starting amounts. Titration of the TRAM antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL,IHC (Paraffin): 0.5-1 μg/mL,IHC (Frozen): 0.5-1 μg/mL,Immunocytochemistry: 0.5-1 μg/mL

Restrictions: For Research Use only

Handling

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: -20 °C

Storage Comment: After reconstitution, the TRAM antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Target Details for TICAM2

Target: TICAM2 (Toll-Like Receptor Adaptor Molecule 2 (TICAM2))

Alternative Name: TRAM (TICAM2 Products)

Synonyms: MyD88-4 antibody, TICAM-2 antibody, TIRAP3 antibody, TIRP antibody, TRAM antibody, B430113A10 antibody, Tirp antibody, Trif antibody, toll like receptor adaptor molecule 2 antibody, toll-like receptor adaptor molecule 2 antibody, TICAM2 antibody, Ticam2 antibody

Background: By crosslinking and reconstitution of canine proteoliposomes, followed by microsequencing and PCR screening of canine kidney and HeLa cell cDNA libraries, Gorlich et al.(1992) isolated cDNAs encoding TRAM (translocating chain-associating membrane protein). The International Radiation Hybrid Mapping Consortium mapped the gene to chromosome 8. Sequence analysis predicted that human TRAM is a 374-amino acid, 8-pass transmembrane protein that shares 95 % amino acid identity with the canine protein. Functional analysis indicated that the protein influences glycosylation and is stimulatory or required for the translocation of secretory proteins.

UniProt: Q15629

Pathways: TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades