The stated application concentrations are suggested starting points. Titration of the NLRP4G antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL,IHC (Paraffin): 0.5-1 μg/mL
Restrictions
For Research Use only
Buffer
0.5 mg/mL if reconstituted with 0.2 mL sterile DI water
Storage
-20 °C
Storage Comment
After reconstitution, the NLRP4G antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
NLRP4G is expressed in mouse ovaries, restricted to the oocytes at various follicular stages and decline with oocyte aging. The protein is oocyte specific, but testing shows it is not required for oocyte maturation, suggesting it plays a role in oogenesis.