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Nuclear Marker antibody

Reactivity: Human, Mouse, Rat FACS, IF, ICC, IHC (p) Host: Mouse Monoclonal NM106 unconjugated
Catalog No. ABIN3026799
  • Target See all Nuclear Marker products
    Nuclear Marker
    Reactivity
    • 5
    • 3
    • 3
    • 2
    Human, Mouse, Rat
    Host
    • 5
    Mouse
    Clonality
    • 5
    Monoclonal
    Conjugate
    • 3
    • 2
    This Nuclear Marker antibody is un-conjugated
    Application
    • 4
    • 4
    • 2
    • 2
    • 1
    • 1
    • 1
    Flow Cytometry (FACS), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    No Cross-Reactivity
    Pig (Porcine)
    Characteristics
    This mAb is an excellent marker for all nuclei in cells in tissues. It is a part of a new panel of reagents, which recognizes subcellular organelles or compartments of cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. This mAb recognizes an antigen associated with the nuclei in all cells. It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear marker in subcellular fractions. It produces a speckled pattern in normal and malignant cells and may be used to stain the nuclei of cells in fixed tissue sections.
    Purification
    Protein G affinity chromatography
    Immunogen
    Nuclei of HL60 cells were used as the immunogen for the Nuclear marker antibody.
    Clone
    NM106
    Isotype
    IgG1 kappa
  • Application Notes
    Optimal dilution of the Nuclear marker antibody should be determined by the researcher.

    1. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10  mM Citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
    2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Immunocytochemistry (Acetone-fixed): 0.25-0.5 μg/mL for 30 min at RT,Immunohistochemistry (FFPE): 0.25-0.5 μg/mL for 30 min at RT (1),Prediluted format: incubate for 30 min at RT (2)

    Restrictions
    For Research Use only
  • Concentration
    1 mg/mL
    Buffer
    1 mg/mL in 1X PBS, BSA free, sodium azide free
    Preservative
    Azide free
    Storage
    4 °C,-20 °C
    Storage Comment
    Store the Nuclear marker antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
  • Target
    Nuclear Marker
    Abstract
    Nuclear Marker Products
    Background
    This mAb is an excellent marker for all nuclei in cells in tissues. It is a part of a new panel of reagents, which recognizes subcellular organelles or compartments of cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. This mAb recognizes an antigen associated with the nuclei in all cells. It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear marker in subcellular fractions. It produces a speckled pattern in normal and malignant cells and may be used to stain the nuclei of cells in fixed tissue sections.
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