ODC1 antibody

Catalog No. ABIN3026477

Product Details anti-ODC1 Antibody

Target: ODC1 (Ornithine Decarboxylase 1 (ODC1))

Reactivity: Human, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This ODC1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Characteristics: This antibody recognizes a 53 kDa protein, identified as the Ornithine Decarboxylase. ODC-1 is the initial and rate-limiting enzyme in the biosynthetic pathway of polyamines and is involved in the conversion of ornithine to putrescine. The biological activity of ODC-1 is rapidly induced in response to virtually all agents known to promote cell proliferation including hormones, drugs, growth factors, mitogens, and tumor promoters. Reportedly, ODC-1 mRNA levels are elevated in lung carcinomas as well as in colon adenomas and carcinomas. Activity in colorectal carcinomas is greater than those in adenomas and normal mucosa.

Purification: Protein G affinity chromatography

Immunogen: Recombinant human ODC-1 protein was used as the immunogen for this antibody.

Clone: ODC1-485

Isotype: IgG1 kappa

Application Details

Application Notes: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. FACS: 0.5-1 μg/million cells,IF: 0.5-1 μg/mL,WB: 0.5-1 μg/mL,IHC (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1),Prediluted format : incubate for 30 min at RT (2)

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the ODC-1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Target Details for ODC1

Target: ODC1 (Ornithine Decarboxylase 1 (ODC1))

Alternative Name: ODC-1 / Ornithine Decarboxylase 1 (ODC1 Products)

Background: This antibody recognizes a 53  kDa protein, identified as the Ornithine Decarboxylase. ODC-1 is the initial and rate-limiting enzyme in the biosynthetic pathway of polyamines and is involved in the conversion of ornithine to putrescine. The biological activity of ODC-1 is rapidly induced in response to virtually all agents known to promote cell proliferation including hormones, drugs, growth factors, mitogens, and tumor promoters. Reportedly, ODC-1 mRNA levels are elevated in lung carcinomas as well as in colon adenomas and carcinomas. Activity in colorectal carcinomas is greater than those in adenomas and normal mucosa.

Gene ID: 4953