CD45RO antibody
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- Target See all CD45RO products
- CD45RO
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Reactivity
- Human, Chimpanzee, Marmoset
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This CD45RO antibody is un-conjugated
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Application
- Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Characteristics
- This antibody recognizes a 180-185 kDa protein, identified as isoform of leukocyte common antigen (CD45) called CD45RO (4th Leucocyte Typing Workshop: Code No. N31). The epitope recognized by this antibody is sensitive to neuraminidase digestion. CD45RO is found on mature activated T-cells, most thymocytes, and a sub-population of resting T-cells within both CD4 and CD8 subsets. The CD45RO antibody shows no reactivity with normal B or natural killer cells, but reacts with granulocytes and monocytes. Reportedly, antibody to CD45RO is useful to identify T-cell lymphomas and leukemias. It rarely stains NK cells or B-cell lymphomas.
- Purification
- Protein G affinity chromatography
- Immunogen
- Cultured human T-cells from an IL-2-dependent T-cell line (CA1) were used as the immunogen for the CD45RO antibody.
- Clone
- UCHL-1
- Isotype
- IgG2a kappa
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anti-CD45RO (CD45RO) antibody
CD45RO Reactivity: Human, Mouse, Rat WB, IHC (p), IF (p) Host: Rabbit Polyclonal unconjugated
anti-CD45RO (CD45RO) antibodyGMP Grade CD45RO Reactivity: Human FACS, IHC, IP Host: Mouse Monoclonal UCHL1 unconjugated
anti-CD45RO (CD45RO) antibody (FITC)GMP Grade CD45RO Reactivity: Human FACS Host: Mouse Monoclonal UCHL1 FITC
anti-CD45RO (CD45RO) antibody (PE)GMP Grade CD45RO Reactivity: Human FACS Host: Mouse Monoclonal UCHL1 PE
anti-CD45RO (CD45RO) antibody (APC)GMP Grade CD45RO Reactivity: Human FACS Host: Mouse Monoclonal UCHL1 APC
anti-CD45RO (CD45RO) antibody (mFluor™450)GMP Grade CD45RO Reactivity: Human FACS Host: Mouse Monoclonal UCHL1 mFluor™450
anti-CD45RO (CD45RO) antibody (PE-CF®594)GMP Grade CD45RO Reactivity: Human FACS Host: Mouse Monoclonal UCHL1 PE-CF®594
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- Application Notes
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The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the CD45RO antibody to be titered up or down for optimal performance.
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. FACS: 0.5-1 μg/million cells,IF: 1-2 μg/mL,IHC (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1),Prediluted format : incubate for 30 min at RT (2) - Restrictions
- For Research Use only
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- Concentration
- 1 mg/mL
- Buffer
- 1 mg/mL in 1X PBS, BSA free, sodium azide free
- Preservative
- Azide free
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store the CD45RO antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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- Target
- CD45RO
- Alternative Name
- CD45RO (CD45RO Products)
- Background
- This antibody recognizes a 180-185 kDa protein, identified as isoform of leukocyte common antigen (CD45) called CD45RO (4th Leucocyte Typing Workshop: Code No. N31). The epitope recognized by this antibody is sensitive to neuraminidase digestion. CD45RO is found on mature activated T-cells, most thymocytes, and a sub-population of resting T-cells within both CD4 and CD8 subsets. The CD45RO antibody shows no reactivity with normal B or natural killer cells, but reacts with granulocytes and monocytes. Reportedly, antibody to CD45RO is useful to identify T-cell lymphomas and leukemias. It rarely stains NK cells or B-cell lymphomas.
- Gene ID
- 5788
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