Recombinant Keratin Acidic (AE1) antibody

Catalog No. ABIN3026306

Product Details

Target: Keratin Acidic (AE1)

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purification: Protein A affinity chromatography

Immunogen: An amino acid sequence common to acidic/type I keratins was used as the immunogen for the Acidic Cytokeratin antibody.

Clone: RMAK1-1

Isotype: IgG kappa

Application Details

Application Notes: Titering of the Acidic Cytokeratin antibody may be required for optimal performance.

1. This antibody will detect cytokeratins -10, -14, -15, -16, -19.
2. FFPE testing is enhanced by boiling sections in  pH 6 10  mM citrate buffer for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.\. Immunofluorescence: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (2)

Restrictions: For Research Use only

Handling

Concentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store the Acidic Cytokeratin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Target Details

Target: Keratin Acidic (AE1)

Alternative Name: Acidic Cytokeratin (AE1 Products)

Background: There are two types of cytokeratins/keratins/CKs: the acidic type I cytokeratins and the basic or neutral type II cytokeratins. The subsets of cytokeratins which an epithelial cell expresses depends mainly on the type of epithelium, the moment in the course of terminal differentiation and the stage of development. Thus this specific keratin fingerprint allows the classification of all epithelia upon their keratin expression profile. Furthermore this applies also to the malignant counterparts of the epithelia (carcinomas), as the keratin profile tends to remain constant when an epithelium undergoes malignant transformation. The main clinical implication is that the study of the keratin profile by immunohistochemistry techniques is a tool of immense value widely used for tumor diagnosis and characterization in surgical pathology. [Wiki]