CLIP antibody

Catalog No. ABIN3026078

Product Details anti-CLIP Antibody

Target: CLIP (Corticotropin-Like Intermediate Peptide (CLIP))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CLIP antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Western Blotting (WB)

Purification: Protein G affinity chromatography

Immunogen: Recombinant full-length human protein was used as the immunogen for the CLIP antibody.

Clone: CLIP-1133

Isotype: IgG1 kappa

Application Details

Application Notes: Optimal dilution of the CLIP antibody should be determined by the researcher.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 1-2 μg/mL,Western blot: 0.5-1 μg/mL

Restrictions: For Research Use only

Handling

Concentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store the CLIP antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Target Details for CLIP

Target: CLIP (Corticotropin-Like Intermediate Peptide (CLIP))

Alternative Name: CLIP (CLIP Products)

Background: Recognizes proteins of 33, 35 and 41  kDa, which are identified as various isoforms of CD74/CLIP. Its epitope is localized in the extracellular domain of CD74. CD74/CLIP is a type II transmembrane protein which binds to the peptide binding groove of newly synthesized MHC class II alpha/beta heterodimers and prevents their premature association with endogenous polypeptides. The molecule plays a critical role in the presentation of peptides, by the MHC class II antigens, to CD4 positive lymphocytes. CD74/CLIP is expressed on MHC class II positive cells including B cells, a subset of activated T cells, monocytes, and dendritic cells and by various types of carcinomas. It is expressed primarily by antigen presenting cells, such as B-lymphocytes (from before the pre-B cell stage to before the plasma cell stage), macrophages, and monocytes, and many epithelial cells. Anti-CD74/CLIP stains predominantly germinal center lymphocytes and B-cell lymphomas, but rarely T-cell lymphomas and has been shown to be useful in differentiating atypical fibroxanthoma (-) from malignant fibrous histiocytoma (+).