MFGE8 antibody

Catalog No. ABIN3025860

Product Details anti-MFGE8 Antibody

Target: MFGE8 (Milk Fat Globule-EGF Factor 8 Protein (MFGE8))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MFGE8 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purification: Protein G affinity chromatography

Immunogen: A human milk fat globule membrane preparation was used as the immunogen for the MFG-E8 antibody.

Clone: SPM291

Isotype: IgG1 kappa

Application Details

Application Notes: Optimal dilution of the MFG-E8 antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Western blot: 1-2 μg/mL, Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 1-2 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1),Prediluted format : incubate for 30 min at RT (2)

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the MFG-E8 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Target Details for MFGE8

Target: MFGE8 (Milk Fat Globule-EGF Factor 8 Protein (MFGE8))

Alternative Name: MFG-E8 (MFGE8 Products)

Synonyms: BA46 antibody, EDIL1 antibody, HMFG antibody, HsT19888 antibody, MFG-E8 antibody, MFGM antibody, OAcGD3S antibody, SED1 antibody, SPAG10 antibody, hP47 antibody, ba46 antibody, edil1 antibody, oacgd3s antibody, MGC75581 antibody, hst19888 antibody, MGC145109 antibody, MFGE8 antibody, mfge8 antibody, zgc:154157 antibody, MGC99228 antibody, AA408458 antibody, AI325141 antibody, Mfgm antibody, P47 antibody, lactadherin antibody, AGS antibody, MFGME8 antibody, MFGMP-E8 antibody, im:6895406 antibody, mfge8l antibody, zgc:110846 antibody, milk fat globule-EGF factor 8 protein antibody, milk fat globule-EGF factor 8 protein b antibody, milk fat globule-EGF factor 8 protein L homeolog antibody, milk fat globule-EGF factor 8 protein a antibody, MFGE8 antibody, mfge8 antibody, mfge8b antibody, mfge8.L antibody, Mfge8 antibody, mfge8a antibody

Background: Recognizes a protein of 40-45  kDa, identified as human milk fat globule membrane protein (HMFG), Lactadherin or MFG-E8. HMFG is present on normal human breast epithelial cells and cell lines derived from breast carcinomas, as well as to the outer surface of the human milk fat globule. HMFG is considered as a differentiation marker. It is useful as specific breast epithelial marker and can also provide a tool to study the role of the cell surface in normal and neoplastic mammary development.

Pathways: SARS-CoV-2 Protein Interactome