Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

HLA-DP/DR antibody

HLA-DP/DR Reactivity: Human FACS, IHC (p), IF Host: Mouse Monoclonal Bra-14 unconjugated
Catalog No. ABIN3025464
  • Target See all HLA-DP/DR products
    HLA-DP/DR (MHC Class II HLA-DP/DR (HLA-DP/DR))
    Reactivity
    • 16
    • 5
    Human
    Host
    • 16
    Mouse
    Clonality
    • 16
    Monoclonal
    Conjugate
    • 4
    • 4
    • 4
    • 2
    • 2
    This HLA-DP/DR antibody is un-conjugated
    Application
    • 16
    • 8
    • 8
    • 4
    • 2
    • 1
    Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)
    Characteristics
    Reacts with a common epitope of human major histocompatibility (MHC) class II antigens, HLA-DR and DP. Human MHC class II antigens are transmembrane glycoproteins composed of an chain (36 kDa) and a chain (27 kDa). They are expressed primarily on antigen presenting cells such as B lymphocytes, monocytes, macrophages, and thymic epithelial cells and are also present on activated T lymphocytes. Human MHC class II genes are located in the HLA-D region that encodes at least six and ten chain genes. Three loci, DR, DQ and DP, encode the major expressed products of the human class II region. The human MHC class II molecules bind intracellularly processed peptides and present them to T-helper cells. They, therefore, have a critical role in the initiation of the immune response. It has been shown that some autoimmune diseases are associated with certain class II alleles.
    Purification
    Protein G affinity chromatography
    Immunogen
    Human REH cells were used as the immunogen for the HLA-DR antibody (HLA-DP).
    Clone
    Bra-14
    Isotype
    IgG3 kappa
  • Application Notes
    Optimal dilution of the HLA-DR antibody (HLA-DP) should be determined by the researcher.

    1. Staining of formalin/paraffin tissues requires boiling tissue sections in 10  mM Citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
    2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1),Prediluted format : incubate for 30 min at RT (2)

    Restrictions
    For Research Use only
  • Concentration
    0.2 mg/mL
    Buffer
    0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-20 °C
    Storage Comment
    Store the HLA-DR antibody (HLA-DP) at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
  • Target
    HLA-DP/DR (MHC Class II HLA-DP/DR (HLA-DP/DR))
    Alternative Name
    HLA-DR (HLA-DP) (HLA-DP/DR Products)
    Background
    Reacts with a common epitope of human major histocompatibility (MHC) class II antigens, HLA-DR and DP. Human MHC class II antigens are transmembrane glycoproteins composed of an chain (36  kDa) and a chain (27  kDa). They are expressed primarily on antigen presenting cells such as B lymphocytes, monocytes, macrophages, and thymic epithelial cells and are also present on activated T lymphocytes. Human MHC class II genes are located in the HLA-D region that encodes at least six and ten chain genes. Three loci, DR, DQ and DP, encode the major expressed products of the human class II region. The human MHC class II molecules bind intracellularly processed peptides and present them to T-helper cells. They, therefore, have a critical role in the initiation of the immune response. It has been shown that some autoimmune diseases are associated with certain class II alleles.
You are here:
Support