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Neurofilament antibody

Reactivity: Human, Rat, Mouse, Chicken, Pig FACS, IHC (p) Host: Mouse Monoclonal NF421 unconjugated
Catalog No. ABIN3025341
  • Target See all Neurofilament products
    Neurofilament
    Reactivity
    • 26
    • 23
    • 20
    • 14
    • 14
    • 3
    Human, Rat, Mouse, Chicken, Pig
    Host
    • 21
    • 7
    • 1
    Mouse
    Clonality
    • 21
    • 8
    Monoclonal
    Conjugate
    • 15
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This Neurofilament antibody is un-conjugated
    Application
    • 17
    • 14
    • 9
    • 7
    • 6
    • 5
    • 2
    • 1
    Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Purification
    Protein G purified Neurofilament antibody
    Immunogen
    Human recombinant Neurofilament protein was used as the immunogen. (1)
    Clone
    NF421
    Isotype
    IgG1
  • Application Notes
    The usable concentrations in the Applications Details are suggestions only. The use of different protocols/secondaries/substrates may require the Neurofilament antibody to be titered for optimal performance.

    1. The NF421 antibody is specific to the heavy subunit.
    2. FFPE tissues requires boiling tissue sections in 10  mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
    3. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. FACS: 0.5-1 μg/million cells,IHC (FFPE): 0.5-1 μg/mL for 30 minutes at RT (2),Prediluted format : incubate for 30 min at RT (3)

    Restrictions
    For Research Use only
  • Concentration
    1 mg/mL
    Buffer
    1 mg/mL in 1X PBS, BSA free, sodium azide free
    Preservative
    Azide free
    Storage
    4 °C,-20 °C
    Storage Comment
    Store the Neurofilament antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
  • Target
    Neurofilament
    Abstract
    Neurofilament Products
    Background
    Neurofilaments make up the main structural elements of axons and dendrites and are found in neurons, peripheral nerves, and sympathetic ganglion cells. Neurofilaments consist of three major subunits with molecular weights of 68  kDa (NF-L), 160  kDa (NF-M) and 200  kDa (NF-H). The fourth neurofilament subunit is alpha internexin in the central nervous system and peripherin in the peripheral nervous system.
    Gene ID
    4744
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