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Calponin antibody

Reactivity: Human, Rat IHC (p), IF, FACS Host: Mouse Monoclonal CNN1-832-CALP unconjugated
Catalog No. ABIN3025302
  • Target See all Calponin products
    Calponin
    Reactivity
    • 38
    • 32
    • 24
    • 8
    • 8
    • 7
    • 5
    • 3
    • 1
    • 1
    • 1
    Human, Rat
    Host
    • 24
    • 15
    Mouse
    Clonality
    • 27
    • 12
    Monoclonal
    Conjugate
    • 24
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This Calponin antibody is un-conjugated
    Application
    • 18
    • 17
    • 17
    • 12
    • 11
    • 10
    • 9
    • 2
    • 1
    • 1
    • 1
    Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Flow Cytometry (FACS)
    Purification
    Protein G affinity chromatography
    Immunogen
    Recombinant full-length human protein (CNN1/832) and crude human uterus extract (CALP) were used as the immunogen for the Calponin antibody cocktail.
    Clone
    CNN1-832-CALP
    Isotype
    IgG
  • Application Notes
    Optimal dilution of the Calponin antibody cocktail should be determined by the researcher.

    1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Tris with 1  mM EDTA,  pH 9.0, for 10-20 min followed by cooling at RT for 20 min
    2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 1-2 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1),Prediluted format : incubate for 30 min at RT (2)

    Restrictions
    For Research Use only
  • Concentration
    1 mg/mL
    Buffer
    1 mg/mL in 1X PBS, BSA free, sodium azide free
    Preservative
    Azide free
    Storage
    4 °C,-20 °C
    Storage Comment
    Store the Calponin antibody cocktail at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
  • Target
    Calponin
    Abstract
    Calponin Products
    Synonyms
    CalPoNin antibody, cpn-1 antibody, cpn-2 antibody
    Background
    Multiple isoelectric variants of calponin have been identified, however only two molecular weight isoforms exist, a 34  kDa form and a 29  kDa form. Expression of the 29  kDa form, I-calponin, is primarily restricted to muscle of the urogenital tract, whereas the higher molecular weight variant has been demonstrated in vascular and visceral smooth muscle. In Western blotting, this mAb reacts with only the 34  kDa form of calponin in extracts of human aortic medial smooth muscle and is unreactive with fibroblast extracts of cultivated human foreskin. Calponin is a calmodulin, F-actin and tropomyosin binding protein, which is thought to be involved in the regulation of smooth muscle contraction. Calponin expression is restricted to smooth muscle cells and has been shown to be a marker of the differentiated (contractile) phenotype of developing smooth muscle.
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