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MAP3K1 antibody (AA 1211-1310)

MAP3K1 Reactivity: Human WB, IF, IHC (p), FACS Host: Mouse Monoclonal 2F6 unconjugated
Catalog No. ABIN3025243
  • Target See all MAP3K1 Antibodies
    MAP3K1 (Mitogen-Activated Protein Kinase Kinase Kinase 1 (MAP3K1))
    Binding Specificity
    • 16
    • 14
    • 12
    • 9
    • 9
    • 6
    • 5
    • 4
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 1211-1310
    Reactivity
    • 68
    • 42
    • 18
    • 5
    • 5
    • 4
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    Human
    Host
    • 86
    • 6
    Mouse
    Clonality
    • 86
    • 6
    Monoclonal
    Conjugate
    • 47
    • 9
    • 7
    • 7
    • 5
    • 5
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    This MAP3K1 antibody is un-conjugated
    Application
    • 52
    • 49
    • 23
    • 15
    • 9
    • 7
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS)
    Purification
    Protein G affinity chromatography
    Immunogen
    A partial recombinant protein (aa 1211-1310) was used as the immunogen for the MAP3K1 antibody.
    Clone
    2F6
    Isotype
    IgG2a kappa
    Top Product
    Discover our top product MAP3K1 Primary Antibody
  • Application Notes
    Optimal dilution of the MAP3K1 antibody should be determined by the researcher.

    1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Tris with 1  mM EDTA,  pH 9.0, for 10-20 min followed by cooling at RT for 20 min
    2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 1-2 μg/mL,Western blot: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1),Prediluted format : incubate for 30 min at RT (2)

    Restrictions
    For Research Use only
  • Concentration
    0.2 mg/mL
    Buffer
    0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-20 °C
    Storage Comment
    Store the MAP3K1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
  • Target
    MAP3K1 (Mitogen-Activated Protein Kinase Kinase Kinase 1 (MAP3K1))
    Alternative Name
    MAP3K1 (MAP3K1 Products)
    Synonyms
    MAP3K1 antibody, Mekk1 antibody, MAPKKK1 antibody, MEKK1 antibody, Mekk antibody, ARAKIN antibody, ATMEKK1 antibody, MAPK/ERK kinase kinase 1 antibody, MAPKKK8 antibody, T15F16.5 antibody, T15F16_5 antibody, MEKK antibody, MEKK 1 antibody, SRXY6 antibody, mitogen-activated protein kinase kinase kinase 1 antibody, mitogen-activated protein kinase kinase kinase 1, E3 ubiquitin protein ligase antibody, MAPK/ERK kinase kinase 1 antibody, MAP3K1 antibody, map3k1 antibody, Map3k1 antibody, MEKK1 antibody
    Background
    Mitogen-activated protein (MAP) kinase cascades are activated by various extracellular stimuli, including growth factors. The MEK kinases (also designated MAP kinase kinase kinases, MKKKs, MAP3Ks or MEKKs) phosphorylate and thereby activate the MEKs (also called MAP kinase kinases or MKKs), including ERK, JNK and p38. These activated MEKs in turn phosphorylate and activate the MAP kinases. The MEK kinases include Raf-1, Raf-B, Mos, MEK kinase-1, MEK kinase-2, MEK kinase-3, MEK kinase-4 and ASK 1 (MEK kinase- 5). MEK kinase-1 activates the ERK and c-Jun NH2-terminal kinase (JNK) pathways by phosphorylation of MAP2K1 and MAP2K4, and also activates the central protein kinases of the NF-kB pathway, CHUK and IKBKB. Additionally, MEK kinase-1 uses an E3 ligase through its PHD domain, a RING-finger-like structure, to target proteins for degradation through ubiquitination.
    Pathways
    MAPK Signaling, Interferon-gamma Pathway, Caspase Cascade in Apoptosis, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Activation of Innate immune Response, Regulation of Actin Filament Polymerization, Toll-Like Receptors Cascades
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