MAML3 antibody
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- Target See all MAML3 Antibodies
- MAML3 (Mastermind-Like 3 (MAML3))
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This MAML3 antibody is un-conjugated
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Application
- Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Purification
- Protein G affinity chromatography
- Immunogen
- A human full length recombinant protein was used as the immunogen for this MAML3 antibody.
- Clone
- MMLP3-1
- Isotype
- IgG1
- Top Product
- Discover our top product MAML3 Primary Antibody
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- Application Notes
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The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the MAML3 antibody to be titered up or down for optimal performance.
1. Staining of FFPE tissue requires boiling sections in 10 mM Tris with 1 mM EDTA, pH 9, for 10-20 min followed by cooling at RT for 20 min.\. FACS: 0.5-1 μg/10^6 cells,IF: 1-2 μg/mL,IHC (FFPE): 0.5-1 μg/mL (1)
- Restrictions
- For Research Use only
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- Concentration
- 0.2 mg/mL
- Buffer
- 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store the MAML3 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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- Target
- MAML3 (Mastermind-Like 3 (MAML3))
- Alternative Name
- MAML3 (MAML3 Products)
- Synonyms
- AV234550 antibody, BC049812 antibody, Mam-2 antibody, mKIAA1816 antibody, Glrp1 antibody, CAGH3 antibody, ERDA3 antibody, GDN antibody, MAM-2 antibody, MAM2 antibody, TNRC3 antibody, mastermind like 3 (Drosophila) antibody, mastermind-like transcriptional coactivator 3 antibody, mastermind like transcriptional coactivator 3 antibody, Maml3 antibody, MAML3 antibody
- Background
- Mastermind-like 3 is a nuclear speckle protein that acts as a transcriptional coactivator for Notch receptors. The Notch signaling pathway influences cell fate by regulating the ability of precursor cells to properly respond to developmental signals. MAML3 is a member of the mastermind-like family of proteins that are human homologs of the Drosophila melanogaster mastermind protein. Through its N-terminal region, MAML3 interacts with the ankyrin repeats of the Notch 1-4 proteins. This interaction leads to formation of a DNA-binding complex with the Notch proteins and RBP-Jκ, a complex that can then induce HES1 gene expression. While the N-terminal domain of MAML3 is essential for proper Notch binding, the C-terminal domain is essential for transcriptional activation. Due to its involvement in cell signaling and transcriptional activation, its upregulation is thought to be involved in oncogenesis.
- Gene ID
- 55534
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