Elastase 3B antibody (AA 82-238)

Catalog No. ABIN3024740

Product Details anti-Elastase 3B Antibody

Target: Elastase 3B (ELA3B) (Elastase 3B, Pancreatic (ELA3B))

Binding Specificity: AA 82-238

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This Elastase 3B antibody is un-conjugated

Application: ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purification: Protein G

Immunogen: A partial recombinant protein (aa 82-238) was used as the immunogen for the Elastase 3B antibody.

Clone: ELTS3B-2

Isotype: IgG1

Application Details

Application Notes: Optimal dilution of the Elastase 3B antibody should be determined by the researcher.

1. FFPE staining requires boiling tissue sections in 10  mM Tris with 1  mM EDTA,  pH 9.0, for 10-20 min followed by cooling at RT for 20 min.\. ELISA: 2-4 μg/mL, order BSA free format for coating,IF: 1-2 μg/mL, IHC-P: 1-2 μg/mL (1)

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the Elastase 3B antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Target Details for Elastase 3B

Target: Elastase 3B (ELA3B) (Elastase 3B, Pancreatic (ELA3B))

Alternative Name: Elastase 3B (ELA3B Products)

Synonyms: CBPP antibody, E1 antibody, EL-1 antibody, ELA3B antibody, 0910001F22Rik antibody, 2310074F01Rik antibody, AI504000 antibody, Ela3 antibody, Ela3b antibody, ela3b antibody, MGC82867 antibody, ELA1 antibody, Cela3b antibody, CELA3B antibody, chymotrypsin like elastase family member 3B antibody, chymotrypsin-like elastase family, member 3B antibody, elastase 3B, pancreatic antibody, proproteinase E antibody, chymotrypsin like elastase family member 3B L homeolog antibody, chymotrypsin-like elastase family member 3B antibody, CELA3B antibody, Cela3b antibody, ela3b antibody, LOC478196 antibody, cela3b.L antibody, LOC100717936 antibody, LOC100071692 antibody

Background: Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six genes which encode the structurally similar proteins: Elastase 1, 2, 2A, 2B, 3A, and 3B. Unlike other elastases, 3B has little elastolytic activity. Like most of the human elastases, 3B is secreted from the pancreas as a zymogen and, like other serine proteases such as trypsin, chymotrypsin and kallikrein, it has a digestive function in the intestine. Elastase 3B preferentially cleaves proteins after alanine residues. It may also function in the intestinal transport and metabolism of cholesterol. Elastases 3A/B have been referred to as Protease E and as Elastase 1, and excretion of this protein in fecal material is frequently used as a measure of pancreatic function in clinical assays.