Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

Melanoma antibody

Reactivity: Human, Horse IHC (p), IF, FACS Host: Mouse Monoclonal NKI-beteb unconjugated
Catalog No. ABIN3024241
  • Target See all Melanoma products
    Melanoma
    Reactivity
    • 4
    • 2
    • 2
    • 1
    Human, Horse
    Host
    • 4
    Mouse
    Clonality
    • 4
    Monoclonal
    Conjugate
    • 4
    This Melanoma antibody is un-conjugated
    Application
    • 3
    • 3
    • 1
    • 1
    • 1
    Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Flow Cytometry (FACS)
    No Cross-Reactivity
    Rat (Rattus)
    Purification
    Protein G affinity chromatography
    Immunogen
    Membranes from a human melanoma metastasis were used as the immunogen for this Melanoma antibody (gp100).
    Clone
    NKI-beteb
    Isotype
    IgG2b kappa
  • Application Notes
    Differences in protocols and secondaries may require the gp100 antibody to be titered up or down for optimal performance.

    1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.\. FACS: 0.5-1 μg/million cells,IF: 1-2 μg/mL,IHC (FFPE): 1-2 μg/mL for 30 minutes at RT (1)

    Restrictions
    For Research Use only
  • Concentration
    1 mg/mL
    Buffer
    1 mg/mL in 1X PBS, BSA free, sodium azide free
    Preservative
    Azide free
    Storage
    4 °C,-20 °C
    Storage Comment
    Store the Melanoma antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
  • Target
    Melanoma
    Abstract
    Melanoma Products
    Background
    By immunohistochemistry, this melanoma gp100 antibody specifically recognizes a protein in melanocytes and melanomas. It reacts with junctional and blue nevus cells and variably with fetal and neonatal melanocytes. Intradermal nevi, normal adult melanocytes, and non-melanocytic cells are negative. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin. The melanoma antibody labels formalin/paraffin melanomas and other tumors showing melanocytic differentiation.
    Gene ID
    6490
You are here:
Support